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膜进样质谱法：藻类研究的强大工具。

Membrane Inlet Mass Spectrometry: A Powerful Tool for Algal Research.

作者信息

Burlacot Adrien, Burlacot François, Li-Beisson Yonghua, Peltier Gilles

机构信息

Aix Marseille Univ, Commissariat à l'énergie Atomique et aux énergies Alternatives (CEA), Centre National de la Recherche Scientifique (CNRS), Institut de Biosciences et Biotechnologies d'Aix- Marseille (BIAM), CEA Cadarache, Saint Paul-Lez-Durance, France.

出版信息

Front Plant Sci. 2020 Sep 4;11:1302. doi: 10.3389/fpls.2020.01302. eCollection 2020.

DOI:10.3389/fpls.2020.01302
PMID:33013952
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7500362/
Abstract

Since the first great oxygenation event, photosynthetic microorganisms have continuously shaped the Earth's atmosphere. Studying biological mechanisms involved in the interaction between microalgae and cyanobacteria with the Earth's atmosphere requires the monitoring of gas exchange. Membrane inlet mass spectrometry (MIMS) has been developed in the early 1960s to study gas exchange mechanisms of photosynthetic cells. It has since played an important role in investigating various cellular processes that involve gaseous compounds (O, CO, NO, or H) and in characterizing enzymatic activities or . With the development of affordable mass spectrometers, MIMS is gaining wide popularity and is now used by an increasing number of laboratories. However, it still requires an important theory and practical considerations to be used. Here, we provide a practical guide describing the current technical basis of a MIMS setup and the general principles of data processing. We further review how MIMS can be used to study various aspects of algal research and discuss how MIMS will be useful in addressing future scientific challenges.

摘要

自第一次大氧化事件以来，光合微生物一直在塑造地球大气。研究微藻和蓝细菌与地球大气相互作用所涉及的生物学机制需要监测气体交换。膜进样质谱法（MIMS）于20世纪60年代初被开发出来，用于研究光合细胞的气体交换机制。从那时起，它在研究涉及气态化合物（氧气、二氧化碳、一氧化氮或氢气）的各种细胞过程以及表征酶活性方面发挥了重要作用。随着价格合理的质谱仪的发展，MIMS越来越受欢迎，现在越来越多的实验室都在使用它。然而，使用它仍需要重要的理论和实际考虑因素。在这里，我们提供一份实用指南，描述MIMS装置的当前技术基础和数据处理的一般原则。我们进一步回顾了MIMS如何用于研究藻类研究的各个方面，并讨论了MIMS在应对未来科学挑战方面将如何发挥作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9c4f/7500362/82ccd7c447c5/fpls-11-01302-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9c4f/7500362/80edd6cd8f1b/fpls-11-01302-g001.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9c4f/7500362/4929c775f8c6/fpls-11-01302-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9c4f/7500362/b8f58862dc05/fpls-11-01302-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9c4f/7500362/c0c0b31f0874/fpls-11-01302-g008.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9c4f/7500362/de1f96bffe77/fpls-11-01302-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9c4f/7500362/6b400c1879cf/fpls-11-01302-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9c4f/7500362/4929c775f8c6/fpls-11-01302-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9c4f/7500362/b8f58862dc05/fpls-11-01302-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9c4f/7500362/c0c0b31f0874/fpls-11-01302-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9c4f/7500362/82ccd7c447c5/fpls-11-01302-g009.jpg

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