Department of Chemistry, Umeå University, Umeå, Sweden.
Department of Chemistry - Ångström, Uppsala University, Uppsala, Sweden.
Methods Mol Biol. 2024;2790:133-148. doi: 10.1007/978-1-0716-3790-6_8.
This chapter compares two different techniques for monitoring photosynthetic O production; the wide-spread Clark-type O electrode and the more sophisticated membrane inlet mass spectrometry (MIMS) technique. We describe how a simple membrane inlet for MIMS can be made out of a commercial Clark-type cell and outline the advantages and drawbacks of the two techniques to guide researchers in deciding which method to use. Protocols and examples are given for measuring O evolution rates and for determining the number of chlorophyll molecules per active photosystem II reaction center.
本章比较了两种监测光合作用 O 生成的不同技术；广泛使用的克拉克型 O 电极和更复杂的膜进样质谱（MIMS）技术。我们描述了如何用商业克拉克型细胞制作简单的 MIMS 膜进样，并概述了两种技术的优缺点，以指导研究人员决定使用哪种方法。给出了测量 O 演化速率和确定每个活性光系统 II 反应中心的叶绿素分子数的方案和实例。
