College of Pharmacy, Woosuk University, Jeonju, 55338, Republic of Korea.
Department of Agro-food Resources, Rural Development Administration, Wanju, 55365, Republic of Korea.
Arch Pharm Res. 2018 Jul;41(7):743-752. doi: 10.1007/s12272-018-1052-0. Epub 2018 Jul 6.
Aging is a key risk factor for many diseases, understanding the mechanism of which is becoming more important for drug development given the fast-growing aging population. In the course of our continued efforts to discover anti-aging natural products, the active constituent 6-shogaol was isolated from Zingiber officinale Roscoe. The chemical structure of 6-shogaol was identified by comparison of its NMR data with literature values. The lifespan-extending effect of 6-shogaol was observed in a dose-dependent manner in Caenorhabditis elegans that has been widely used as a model organism for human aging studies. Mechanism of such action was investigated using C. elegans models, suggesting that 6-shogaol is capable of increasing stress tolerances via enzyme induction. The proposed mechanism was further supported by observation of the increase in SOD and HSP expressions upon treatment with 6-shogaol in transgenic strains of C. elegans which contain GFP-based reporters. In addition, the mechanism was elaborated by confirming that the effect observed for 6-shogaol is independent from other aging-related factors that are known to affect the aging process of C. elegans.
衰老是许多疾病的关键风险因素，考虑到快速增长的老龄化人口，了解其发病机制对于药物开发变得越来越重要。在我们不断努力发现抗衰老天然产物的过程中，从姜科植物姜中分离出了活性成分 6-姜酚。通过将其 NMR 数据与文献值进行比较，确定了 6-姜酚的化学结构。6-姜酚在秀丽隐杆线虫中具有剂量依赖性的延长寿命作用，秀丽隐杆线虫已被广泛用作人类衰老研究的模式生物。使用秀丽隐杆线虫模型研究了这种作用的机制，表明 6-姜酚能够通过诱导酶来提高应激耐受力。通过观察用 6-姜酚处理含有 GFP 报告基因的秀丽隐杆线虫转基因株系后 SOD 和 HSP 表达的增加，进一步支持了该机制。此外，通过确认观察到的 6-姜酚的作用独立于其他已知影响秀丽隐杆线虫衰老过程的与衰老相关的因素，进一步阐述了该机制。
