Huang Dan-hua, Qi Xue-yong, Ge Yan-ru, Shen Song
Yao Xue Xue Bao. 2017 Mar;52(3):481-7.
In this study, water-dispersible magnetic iron oxide (Fe3O4) nanoparticles were synthesized with solvothermal method. The nanoparticles were characterized with a transmission electron microscopy (TEM) and vibrating sample magnetometer (VSM). The in vitro magnetic resonance response and photothermal conversion characteristics of the nanoparticles were evaluated. In addition, the cellular uptake, cytotoxicity and biodistribution were studied. Finally, magnetic resonance/photothermal dual-modal imaging effect of the as-synthesized Fe3O4 nanoparticles was investigated in the tumor-bearing mice. The results showed that the obtained magnetic nanoparticles were uniform with a mean diameter of about 125 nm. Moreover, the superparamagnetic Fe3O4 nanoparticles showed remarkable magnetic resonance response and photothermal conversion properties. The results of cellular experiments showed that the cell viability was nearly 85% even the concentration of the nanoparticles was up to 1 000 μg·mL−1, an indicator of good biocompatibility. In addition, the nanoparticles could be taken up by the tumor cells and then located in the cytoplasm. After intravenous injection, the nanoparticles were tended to enrich in the tumor over time, which is helpful in achieving dual-modal magnetic resonance/photothermal imaging. In sum, the obtained Fe 3O4 nanoparticles showed great potential to be applied for multi-modal bio-imaging which may play an important role in the diagnosis of tumors.
在本研究中，采用溶剂热法合成了水分散性磁性氧化铁（Fe3O4）纳米颗粒。通过透射电子显微镜（TEM）和振动样品磁强计（VSM）对纳米颗粒进行了表征。评估了纳米颗粒的体外磁共振响应和光热转换特性。此外，还研究了细胞摄取、细胞毒性和生物分布。最后，在荷瘤小鼠中研究了所合成的Fe3O4纳米颗粒的磁共振/光热双模态成像效果。结果表明，所获得的磁性纳米颗粒均匀，平均直径约为125 nm。此外，超顺磁性Fe3O4纳米颗粒表现出显著的磁共振响应和光热转换特性。细胞实验结果表明，即使纳米颗粒浓度高达1000μg·mL−1，细胞活力仍接近85%，这是良好生物相容性的一个指标。此外，纳米颗粒可被肿瘤细胞摄取并定位在细胞质中。静脉注射后，纳米颗粒随时间推移倾向于在肿瘤中富集，这有助于实现双模态磁共振/光热成像。总之，所获得的Fe3O4纳米颗粒在多模态生物成像应用方面显示出巨大潜力，这可能在肿瘤诊断中发挥重要作用。
