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茴香脑对人间充质干细胞成脂分化的mTOR和ROS调控

mTOR and ROS regulation by anethole on adipogenic differentiation in human mesenchymal stem cells.

作者信息

Rhee Yun-Hee, Moon Jeong Hwan, Mo Ji-Hun, Pham Tiffany, Chung Phil-Sang

机构信息

Beckman Laser Institute Korea, Dankook University, 119 Dandae-ro, Cheonan, 31116, Republic of Korea.

Laser Translational Clinical Trial Center, Dankook University Hospital, Cheonan, 31116, Republic of Korea.

出版信息

BMC Cell Biol. 2018 Jul 6;19(1):12. doi: 10.1186/s12860-018-0163-2.

DOI:10.1186/s12860-018-0163-2
PMID:29980168
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6035441/
Abstract

BACKGROUND

Adipocyte differentiation of human mesenchymal stem cells (hMSCs) is dependent on mitochondrial metabolism and reactive oxygen species (ROS) to initiate adipocyte differentiation. Although anethole has been known as an anti-oxidant and lipid peroxidation inhibitor, there is little investigated about its role in adipogenic differentiation.

METHODS

The effects on cytotoxicity and proliferation of anethole in hMSCs were measured by the MTT assay. The anti-adipogenic effect of anethole on hMSCs was analyzed by Oil Red O staining and western blot analysis. The anti-oxidant activity of anethole on hMSC was assessed by flowcytometry and fluorescence staining using 2',7' -dichlorofluorescin diacetate (DCFDA). The western blotting was used to detect of phospho-Akt, phospho-mTOR, phospho-p70S6K, PPARγ, and phsopho-AMP-activated kinase (AMPK).

RESULTS

Anethole suppressed the adipogenic differentiation of hMSCs through down-regulation of Akt-mTOR-p70S6K-PPARγ and up-regulation of AMPK. Anethole affected oxidative conditions through ROS generation. Anethole also rescued AMPK activity and reduced activation of mTOR-p70S6K-PPARγ under oxidative conditions in presence of exogenous hydrogen peroxide.

CONCLUSION

ROS and mTOR regulation is a crucial factor in adipogenic differentiation, anethole has an important role in regulating activities of mTOR/PPARγ and ROS control in adipogenic differentiation of hMSCs.

摘要

背景

人间充质干细胞（hMSCs）的脂肪细胞分化依赖于线粒体代谢和活性氧（ROS）来启动脂肪细胞分化。尽管茴香脑已知是一种抗氧化剂和脂质过氧化抑制剂，但其在脂肪生成分化中的作用鲜少被研究。

方法

通过MTT法检测茴香脑对hMSCs细胞毒性和增殖的影响。通过油红O染色和蛋白质印迹分析来分析茴香脑对hMSCs的抗脂肪生成作用。使用二氯荧光素二乙酸酯（DCFDA）通过流式细胞术和荧光染色评估茴香脑对hMSC的抗氧化活性。蛋白质印迹法用于检测磷酸化Akt、磷酸化mTOR、磷酸化p70S6K、PPARγ和磷酸化AMP激活的蛋白激酶（AMPK）。

结果

茴香脑通过下调Akt-mTOR-p70S6K-PPARγ和上调AMPK来抑制hMSCs的脂肪生成分化。茴香脑通过ROS生成影响氧化条件。在存在外源性过氧化氢的氧化条件下，茴香脑还能恢复AMPK活性并降低mTOR-p70S6K-PPARγ的激活。

结论

ROS和mTOR调节是脂肪生成分化的关键因素，茴香脑在调节hMSCs脂肪生成分化中mTOR/PPARγ的活性和ROS控制方面具有重要作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a50e/6035441/bca600f8f9ca/12860_2018_163_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a50e/6035441/7db4bba1cc63/12860_2018_163_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a50e/6035441/3c01e9b5308d/12860_2018_163_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a50e/6035441/08f760e1a180/12860_2018_163_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a50e/6035441/bca600f8f9ca/12860_2018_163_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a50e/6035441/7db4bba1cc63/12860_2018_163_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a50e/6035441/3c01e9b5308d/12860_2018_163_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a50e/6035441/08f760e1a180/12860_2018_163_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a50e/6035441/bca600f8f9ca/12860_2018_163_Fig4_HTML.jpg

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本文引用的文献

1
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Biomed Pharmacother. 2017 Feb;86:213-220. doi: 10.1016/j.biopha.2016.12.014. Epub 2016 Dec 19.
2
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Adv Exp Med Biol. 2016;929:247-267. doi: 10.1007/978-3-319-41342-6_11.
3
mTOR remains unchanged in diet-resistant (DR) rats despite impaired LKB1/AMPK cascade in adipose tissue.尽管脂肪组织中LKB1/AMPK级联受损，但mTOR在饮食抵抗（DR）大鼠中保持不变。
Dietary anethole: a systematic review of its protective effects against metabolic syndrome.
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J Diabetes Metab Disord. 2023 Dec 27;23(1):619-631. doi: 10.1007/s40200-023-01322-1. eCollection 2024 Jun.
4
NADPH-dependent ROS accumulation contributes to the impaired osteogenic differentiation of periodontal ligament stem cells under high glucose conditions.NADPH 依赖性 ROS 积累导致高糖条件下牙周韧带干细胞成骨分化受损。
Front Endocrinol (Lausanne). 2023 Jun 7;14:1152845. doi: 10.3389/fendo.2023.1152845. eCollection 2023.
5
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J Anim Sci Biotechnol. 2023 Feb 22;14(1):32. doi: 10.1186/s40104-022-00824-x.
6
-anethole Ameliorates Intestinal Injury Through Activation of Nrf2 Signaling Pathway in Subclinical Necrotic Enteritis-Induced Broilers.茴香脑通过激活亚临床坏死性肠炎诱导肉鸡的Nrf2信号通路改善肠道损伤。
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10
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Onco Targets Ther. 2019 Nov 14;12:9685-9696. doi: 10.2147/OTT.S209937. eCollection 2019.
Biochem Biophys Res Commun. 2016 Aug 5;476(4):333-339. doi: 10.1016/j.bbrc.2016.05.123. Epub 2016 May 25.
4
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5
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6
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7
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8
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9
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10
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Mol Nutr Food Res. 2014 Mar;58(3):569-79. doi: 10.1002/mnfr.201300157. Epub 2013 Nov 11.