Yan Dongmei, Tang Botao, Yan Lixin, Zhang Lei, Miao Meijuan, Chen Xi, Sui Guangyi, Zhang Qi, Liu Daoyuan, Wang Hui
Department of Blood Transfusion, The Second Affiliated Hospital of Harbin Medical University, Harbin, People's Republic of China.
Department of Cardiology, Heilongjiang Red Cross Hospital, Harbin, People's Republic of China.
Onco Targets Ther. 2019 Nov 14;12:9685-9696. doi: 10.2147/OTT.S209937. eCollection 2019.
Sodium selenite (NaSeO) has been known to restore the antioxidant capacity of bone marrow mesenchymal stem cells (BMSCs), reduce the production of reactive oxygen species (ROS) in the cells, and promote cell proliferation and inhibit cell apoptosis. However, it is still not clear whether selenium can mediate the differentiation and inhibit the induced hemagglutination of BMSCs. In this study, we attempted to explore the effect of NaSeO on these aspects of BMSCs.
We evaluated the fate of the MSCs isolated from the bone marrow of mice by studying their differentiation and proliferation after treatment with NaSeO. We also simultaneously evaluated the coagulation reaction induced by NaSeOtreated BMSCs in vitro.
While the mice-derived BMSCs expressed CD44, CD73, CD90, and CD105, they did not express CD45. The morphology of the derived cells was homogeneously elongated. These results showed that the isolated cells are indeed BMSCs. We found that 0.1 μM and 1 μM of NaSeO promoted the proliferation and apoptosis of BMSCs, respectively. This showed that NaSeO can be toxic and exert certain side effects on the BMSCs. The results of the osteogenic and adipogenic assay showed that 0.1 μM NaSeO could significantly promote the osteogenic and adipogenic differentiation of BMSCs by upregulating the lipid factors (LPL and PPRAG) and osteogenic factors, RUNX2, COL1, and BGP, in a concentration-dependent manner. Coagulation experiments in animals (mice and rats) revealed that NaSeO can reduce the coagulation time of BMSCs in a concentration-dependent manner, which is related to the high expression of hematopoietic factors (SDF-1α, GM-CSF, IL-7, IL-8, IL-11, and SCF).
NaSeO promotes the proliferation and differentiation as well as reduces the coagulation time of BMSCs, and this effect might enhance the therapeutic effect of BMSCs.
已知亚硒酸钠（NaSeO）可恢复骨髓间充质干细胞（BMSCs）的抗氧化能力，减少细胞中活性氧（ROS）的产生，促进细胞增殖并抑制细胞凋亡。然而，硒是否能介导BMSCs的分化并抑制其诱导的血凝作用仍不清楚。在本研究中，我们试图探究亚硒酸钠对BMSCs这些方面的影响。
我们通过研究用亚硒酸钠处理后小鼠骨髓分离的间充质干细胞（MSCs）的分化和增殖情况，评估其命运。我们还同时评估了经亚硒酸钠处理的BMSCs在体外诱导的凝血反应。
从小鼠分离的BMSCs表达CD44、CD73、CD90和CD105，但不表达CD45。所分离细胞的形态均匀细长。这些结果表明所分离的细胞确实是BMSCs。我们发现0.1μM和1μM的亚硒酸钠分别促进了BMSCs的增殖和凋亡。这表明亚硒酸钠可能具有毒性并对BMSCs产生一定的副作用。成骨和成脂检测结果表明，0.1μM亚硒酸钠可通过浓度依赖性上调脂质因子（LPL和PPRAG）和成骨因子RUNX2、COL1和BGP，显著促进BMSCs的成骨和成脂分化。动物（小鼠和大鼠）凝血实验表明，亚硒酸钠可浓度依赖性地缩短BMSCs的凝血时间，这与造血因子（SDF-1α、GM-CSF、IL-7、IL-8、IL-11和SCF）的高表达有关。
亚硒酸钠促进BMSCs的增殖和分化，并缩短其凝血时间，这种作用可能增强BMSCs的治疗效果。
