Key Laboratory of Bioactive Substances and Resource Utilization of Chinese Herbal Medicine, Ministry of Education, Beijing Key Laboratory of Innovative Drug Discovery of Traditional Chinese Medicine (Natural Medicine) and Translational Medicine, Key Laboratory of Efficacy Evaluation of Chinese Medicine against Glycolipid Metabolic Disorders, State Administration of Traditional Chinese Medicine, Institute of Medicinal Plant Development, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100193, China.
Yunnan Branch, Institute of Medicinal Plant, Chinese Academy of Medical Sciences, Peking Union Medical College, Jinghong 666100, China.
Fitoterapia. 2018 Sep;129:203-209. doi: 10.1016/j.fitote.2018.07.003. Epub 2018 Jul 5.
Nine new polyoxypregnane glycosides, obcordatas A-I (1-9), were isolated from Aspidopterys obcordata Hemsl vines. Their structures were elucidated by extensive spectroscopic methods. Separated compounds were evaluated for antitumor activities against the human cancer cell lines AGS, SW480, HuH-7 and MCF-7, and compounds 1-6 and 9 showed selective cytotoxicity against HuH-7 cells with IC values of 8.7, 10.2, 7.5, 12.1, 16.5, 14.3, and 17.4 μM, respectively. Flow cytometry experiments showed that the effects of compound 1 on the cell cycle were attributable to cell cycle arrest at G1/S phase.
从扁担藤藤茎中分离得到了 9 个新的甾体糖苷化合物，命名为 obcordatas A-I（1-9）。通过广泛的光谱方法阐明了它们的结构。对分离得到的化合物进行了抗肿瘤活性评价，对人胃癌细胞株 AGS、SW480、HuH-7 和 MCF-7 进行了评价，化合物 1-6 和 9 对 HuH-7 细胞表现出选择性细胞毒性，IC 值分别为 8.7、10.2、7.5、12.1、16.5、14.3 和 17.4 μM。流式细胞术实验表明，化合物 1 对细胞周期的影响归因于 G1/S 期的细胞周期阻滞。
