Wieraszko A, Seifert W
Brain Res. 1985 Oct 14;345(1):159-64. doi: 10.1016/0006-8993(85)90847-9.
Rat hippocampal slices were incubated with neuraminidase from Vibrio Cholerae. This enzyme liberates sialic acid from polysialogangliosides converting them into monosialoganglioside GM1. Thus, the tissue is enriched in GM1 content. Another set of slices was incubated with GM1 itself. Both treatments increased the magnitude of potentiation of synaptic response recorded from pyramidal cell layer following high frequency stimulation of Schaffer collateral-commissural fibers. It is concluded that enrichment of synaptic membranes in GM1 enhances the ability of these nerve endings to be potentiated.
将大鼠海马切片与霍乱弧菌神经氨酸酶一起孵育。这种酶从多唾液酸神经节苷脂中释放唾液酸，将它们转化为单唾液酸神经节苷脂GM1。因此，组织中的GM1含量增加。另一组切片与GM1本身一起孵育。在高频刺激海马联合纤维后，这两种处理都增加了从锥体细胞层记录到的突触反应的增强幅度。得出的结论是，突触膜中GM1的富集增强了这些神经末梢的增强能力。
