Department of Medical biochemistry and Molecular Biology, Faculty of Medicine, Cairo University, Cairo, Egypt.
Rheumatology and Rehabilitation Department, Kasr Al-Ainy Hospital, Cairo University, Cairo, Egypt.
Clin Rheumatol. 2019 Feb;38(2):397-402. doi: 10.1007/s10067-018-4191-2. Epub 2018 Jul 10.
Behcet's disease is a chronic, multisystem, inflammatory disease of unknown etiology. Oral ulcers, genital ulcers, cutaneous lesions, and ocular and articular involvement are the prominent features of the disease. The aim of the study was to assess expression of microRNA-146a and its gene polymorphism in Egyptian Behcet's disease (BD) patients and to evaluate their possible relation with clinical manifestations and activity. This is a case-control study, included 47 BD Egyptian patients, recruited from the Rheumatology outpatient clinic, Kasr Alainy Hospital, Cairo University Hospitals, and 50 healthy controls. BD activity was assessed using the BD Current Activity Score. Quantitative expressions of serum microRNA-146a and microRNA-146a rs2910164 SNP genotyping were performed by real-time polymerase chain reaction (RT-PCR). P values < 0.05 were considered statistically significant. Serum microRNA-146a expression was significantly higher in BD patients than in controls (7. 27 ± 4.11, 1.13 ± .37) (P < 0.001). There was a significant association between microRNA-146a expression and eye activity (P = 0.033) and vascular activity (P = 0.041). miRNA-146a rs2910164 genotyping revealed that the frequency of CC genotype was higher in controls (12 vs 8.5%) and the frequency of GG genotype of rs2910164 was higher in the BD patients (59.6 vs 24%) (P = 0.138). MicroRNA-146a expression in Egyptian BD patients is significantly higher than that in controls; there is significant association between microRNA-146a expression and eye and vascular activity of BD. The frequency of CC genotype of rs2910164 was decreased; frequency of GG genotype of rs2910164 was increased in BD patients as compared to controls, suggesting that GG genotype of rs2910164 confers susceptibility to BD while CC genotype has a protective role against BD development.
贝赫切特病是一种病因不明的慢性、多系统、炎症性疾病。口腔溃疡、生殖器溃疡、皮肤损伤以及眼部和关节受累是该病的突出特征。本研究旨在评估埃及贝赫切特病（BD）患者中 microRNA-146a 的表达及其基因多态性，并评估其与临床表现和活动的可能关系。这是一项病例对照研究，纳入了 47 名来自开罗大学医院 Kasr Alainy 风湿病门诊的埃及 BD 患者和 50 名健康对照。使用 BD 现患活动评分评估 BD 活动。通过实时聚合酶链反应（RT-PCR）检测血清 microRNA-146a 和 microRNA-146a rs2910164 SNP 基因分型的定量表达。P 值 < 0.05 被认为具有统计学意义。BD 患者的血清 microRNA-146a 表达明显高于对照组（7.27 ± 4.11，1.13 ± 0.37）（P < 0.001）。microRNA-146a 表达与眼部活动（P = 0.033）和血管活动（P = 0.041）之间存在显著相关性。miRNA-146a rs2910164 基因分型显示，CC 基因型在对照组中的频率较高（12 对 8.5%），而 GG 基因型在 BD 患者中的频率较高（59.6 对 24%）（P = 0.138）。埃及 BD 患者的 microRNA-146a 表达明显高于对照组；microRNA-146a 表达与 BD 的眼部和血管活动之间存在显著相关性。rs2910164 的 CC 基因型频率降低；rs2910164 的 GG 基因型频率在 BD 患者中增加，提示 rs2910164 的 GG 基因型易患 BD，而 CC 基因型对 BD 的发生具有保护作用。
