Department of Orthopaedic Surgery, Beaumont Health, 3601 W. 13 Mile Rd, Royal Oak, MI, 48073, USA.
Department of Orthopaedic Surgery, Oakland University-William Beaumont School of Medicine, 2200 N Squirrel Rd, Rochester, MI, 48309, USA.
J Mater Sci Mater Med. 2018 Jul 10;29(7):109. doi: 10.1007/s10856-018-6117-6.
Percutaneous doxycycline for treatment for aneurysmal bone cysts (ABCs) has been shown to decrease recurrence rates, however, this requires multiple procedures, includes the risks soft tissue necrosis, and does not provide structural support. We propose utilizing curettage with doxycycline-loaded calcium phosphate cement. This study aimed to evaluate the elution profile of doxycycline from calcium phosphate cement. Calcium phosphate cement underwent an in vitro elution protocol evaluating doxycycline concentrations of 0, 5, 10, and 15 mg/mL. Eluted concentrations were quantified utilizing high performance liquid chromatography at predetermined time points over 96 h. Compressive strength was evaluated both pre- and post-elution and micro-computed tomography was utilized to assess changes in cement porosity. Cement with 15 mg/mL of doxycycline maintained a higher average concentration (mean, 95% confidence intervals) (14.5 µg/mL [9.2-19.9 µg/mL]) compared to both 5 mg/mL (5.8 µg/mL [3.1-8.6 µg/mL]; P < 0.001) and 10 mg/mL (8.4 ± µg/mL [6.0-10.9 µg/mL]; P < 0.001). Ultimate stress significantly decreased between pre- and post-elution samples for 10 mg/mL (P= 0.001) and 15 mg/mL (P = 0.004) groups. This study demonstrated a dose-dependent response in ultimate strength and compressive modulus with addition of doxycycline to calcium phosphate cement.
经皮多西环素治疗骨囊肿（ABC）已被证实可降低复发率，但需要多次治疗，存在软组织坏死风险，且无法提供结构支撑。我们建议使用载多西环素磷酸钙骨水泥进行刮除术。本研究旨在评估磷酸钙骨水泥中多西环素的洗脱曲线。磷酸钙骨水泥进行了体外洗脱方案，评估了 0、5、10 和 15 mg/mL 的多西环素浓度。在 96 小时内的预定时间点，利用高效液相色谱法定量洗脱的浓度。在洗脱前后评估抗压强度，并利用微计算机断层扫描评估水泥孔隙率的变化。15 mg/mL 多西环素的水泥保持较高的平均浓度（平均值，95%置信区间）（14.5μg/mL[9.2-19.9μg/mL]），明显高于 5 mg/mL（5.8μg/mL[3.1-8.6μg/mL]；P<0.001）和 10 mg/mL（8.4±μg/mL[6.0-10.9μg/mL]；P<0.001）。10 mg/mL（P=0.001）和 15 mg/mL（P=0.004）组的抗压强度在洗脱前后的样本之间显著降低。本研究表明，在向磷酸钙骨水泥中添加多西环素时，其最终强度和抗压模量呈剂量依赖性。
