Stroobant P, Gullick W J, Waterfield M D, Rozengurt E
EMBO J. 1985 Aug;4(8):1945-9. doi: 10.1002/j.1460-2075.1985.tb03875.x.
Fibroblast-derived growth factor (FDGF), a polypeptide secreted by an SV40-transformed baby hamster kidney cell line (SV28), was purified approximately 1000-fold from SV28-conditioned medium. FDGF, which gave a single band on SDS-polyacrylamide gel electrophoresis, is a hydrophobic and cationic protein of apparent mol. wt. 31 000 containing disulphide-linked polypeptides. This factor is positive in Western blots using human platelet-derived growth factor (PDGF) anti-serum. FDGF is a potent mitogen for Swiss 3T3 cells; half-maximal stimulation of DNA synthesis was achieved at a concentration of approximately 1 nM, comparable with those for human and porcine PDGF. FDGF inhibits EGF binding to Swiss 3T3 cells, as does PDGF. The coincidence of the physical, biological and immunological characteristics of FDGF and PDGF strongly suggests that they are closely related in structure.
成纤维细胞衍生生长因子（FDGF）是由SV40转化的幼仓鼠肾细胞系（SV28）分泌的一种多肽，从SV28条件培养基中纯化了约1000倍。FDGF在SDS-聚丙烯酰胺凝胶电泳上呈现单一条带，是一种表观分子量为31000的疏水阳离子蛋白，含有二硫键连接的多肽。该因子在使用人血小板衍生生长因子（PDGF）抗血清的蛋白质免疫印迹中呈阳性。FDGF是瑞士3T3细胞的有效促有丝分裂原；在浓度约为1 nM时可实现DNA合成的半最大刺激，这与人和猪PDGF相当。FDGF与PDGF一样，可抑制表皮生长因子（EGF）与瑞士3T3细胞的结合。FDGF和PDGF在物理、生物学和免疫学特性上的一致性强烈表明它们在结构上密切相关。
