Chang Sunny C, Ren Shuxun, Rau Christoph D, Wang Jessica J
Division of Cardiology, Department of Medicine, David Geffen School of Medicine at UCLA, Los Angeles, CA, USA.
Methods Mol Biol. 2018;1816:207-220. doi: 10.1007/978-1-4939-8597-5_16.
Isoproterenol is used widely for inducing heart failure in mice. Isoproterenol is a nonselective beta-adrenergic agonist. The acute model mimics stress-induced cardiomyopathy. The chronic model mimics advanced heart failure in humans. In this chapter, we describe a protocol that we used to induce heart failure in 100+ strains of inbred mice. Techniques on surgical pump implantation and echocardiography are described in detail. We also discuss the impact of drug dosage, duration, mortality, age, gender, and strain on cardiac remodeling responses. The success of model creation may be assessed by echocardiogram or molecular markers. This chapter may be relevant to those who are interested in using this heart failure model.
异丙肾上腺素被广泛用于诱导小鼠心力衰竭。异丙肾上腺素是一种非选择性β-肾上腺素能激动剂。急性模型模拟应激性心肌病。慢性模型模拟人类晚期心力衰竭。在本章中，我们描述了一种用于在100多种近交系小鼠中诱导心力衰竭的方案。详细介绍了手术泵植入和超声心动图技术。我们还讨论了药物剂量、持续时间、死亡率、年龄、性别和品系对心脏重塑反应的影响。模型创建的成功与否可通过超声心动图或分子标志物进行评估。本章可能与那些对使用这种心力衰竭模型感兴趣的人相关。
