State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, 2 Sipailou Road, Nanjing 210096, PR China.
State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, 2 Sipailou Road, Nanjing 210096, PR China.
J Colloid Interface Sci. 2018 Nov 15;530:511-520. doi: 10.1016/j.jcis.2018.06.041. Epub 2018 Jun 22.
Reduced glutathione (GSH) is a crucial endogenous antioxidant that plays a central role in maintaining cell activity by involving in cellular defense against reactive oxygen species (ROS). Therefore, the detection of intracellular GSH is essential to monitor the cellular state. Herein, graphene oxide-MnO-fluorescein (GO-MnO-FL) nanocomposite was synthesized and used for GSH detection. GSH could reduce MnO to Mn and block the energy transfer between FL and GO-MnO, leading to the significant fluorescence enhancement of FL. The fluorescence-based GSH sensing has a limit of detection of 1.53 μM and a wide detection range of 10 μM-2 mM. Moreover, the GO-MnO-FL nanocomposite was found to selectively image the cancer cells since the GSH content of cancer cells is higher than that of normal cells. In vivo experiments also confirmed the normal/cancer cell differentiation capability and excellent tumor-targeting ability of the GO-MnO-FL nanocomposite. These properties make the GO-MnO-FL nanocomposite an excellent fluorescent probe for GSH detection both in vitro and in vivo, and we believe it may hold great potential for GSH-mediated cancer diagnosis.
还原型谷胱甘肽（GSH）是一种重要的内源性抗氧化剂，通过参与细胞对活性氧（ROS）的防御，在维持细胞活性方面发挥着核心作用。因此，检测细胞内 GSH 对于监测细胞状态至关重要。在此，合成了氧化石墨烯-氧化锰-荧光素（GO-MnO-FL）纳米复合材料，并用于 GSH 的检测。GSH 可以将 MnO 还原为 Mn，并阻断 FL 和 GO-MnO 之间的能量转移，导致 FL 的荧光显著增强。基于荧光的 GSH 传感具有 1.53μM 的检测限和 10μM-2mM 的宽检测范围。此外，GO-MnO-FL 纳米复合材料被发现可以选择性地对癌细胞进行成像，因为癌细胞中的 GSH 含量高于正常细胞。体内实验也证实了 GO-MnO-FL 纳米复合材料具有正常/癌细胞分化能力和优异的肿瘤靶向能力。这些特性使 GO-MnO-FL 纳米复合材料成为体外和体内 GSH 检测的一种优秀荧光探针，我们相信它在 GSH 介导的癌症诊断方面可能具有巨大的潜力。
