与共有启动子序列同源性增加的乳糖操纵子上游启动子突变体。
lac Up-promoter mutants with increased homology to the consensus promoter sequence.
作者信息
Mandecki W, Goldman R A, Powell B S, Caruthers M H
出版信息
J Bacteriol. 1985 Dec;164(3):1353-5. doi: 10.1128/jb.164.3.1353-1355.1985.
Abstract
Four lac promoter mutants were constructed. The mutations increased the homology between the lac promoter and the consensus promoter sequences by introducing the consensus -10 and -35 regions and the consensus spacing of 17 residues between these two regions. The promoter mutants were cloned into a pBR322-derivatized vector upstream from the lacZ gene, and levels of beta-galactosidase were an indication of promoter activity. All mutants exhibited higher activity than did the wild-type promoter.
摘要
构建了四个乳糖启动子突变体。这些突变通过引入共有-10和-35区域以及这两个区域之间17个残基的共有间距，增加了乳糖启动子与共有启动子序列之间的同源性。将启动子突变体克隆到位于lacZ基因上游的pBR322衍生载体中，β-半乳糖苷酶的水平是启动子活性的指标。所有突变体都表现出比野生型启动子更高的活性。
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