与共有启动子序列同源性增加的乳糖操纵子上游启动子突变体。
lac Up-promoter mutants with increased homology to the consensus promoter sequence.
作者信息
Mandecki W, Goldman R A, Powell B S, Caruthers M H
出版信息
J Bacteriol. 1985 Dec;164(3):1353-5. doi: 10.1128/jb.164.3.1353-1355.1985.
Abstract
Four lac promoter mutants were constructed. The mutations increased the homology between the lac promoter and the consensus promoter sequences by introducing the consensus -10 and -35 regions and the consensus spacing of 17 residues between these two regions. The promoter mutants were cloned into a pBR322-derivatized vector upstream from the lacZ gene, and levels of beta-galactosidase were an indication of promoter activity. All mutants exhibited higher activity than did the wild-type promoter.
摘要
构建了四个乳糖启动子突变体。这些突变通过引入共有-10和-35区域以及这两个区域之间17个残基的共有间距，增加了乳糖启动子与共有启动子序列之间的同源性。将启动子突变体克隆到位于lacZ基因上游的pBR322衍生载体中，β-半乳糖苷酶的水平是启动子活性的指标。所有突变体都表现出比野生型启动子更高的活性。
相似文献
1
lac Up-promoter mutants with increased homology to the consensus promoter sequence.与共有启动子序列同源性增加的乳糖操纵子上游启动子突变体。
J Bacteriol. 1985 Dec;164(3):1353-5. doi: 10.1128/jb.164.3.1353-1355.1985.
2
Mutants of the lac promoter with large insertions and deletions between the CAP binding site and the -35 region.在CAP结合位点与-35区域之间存在大的插入和缺失的乳糖启动子突变体。
Gene. 1984 Nov;31(1-3):263-7. doi: 10.1016/0378-1119(84)90219-1.
3
A lac promoter with a changed distance between -10 and -35 regions.一种在-10区和-35区之间距离发生改变的乳糖启动子。
Nucleic Acids Res. 1982 Feb 11;10(3):903-12. doi: 10.1093/nar/10.3.903.
4
Azorhizobium caulinodans nitrogen fixation (nif/fix) gene regulation: mutagenesis of the nifA -24/-12 promoter element, characterization of a ntrA(rpoN) gene, and derivation of a model.茎瘤固氮根瘤菌的固氮（nif/fix）基因调控：nifA -24/-12启动子元件的诱变、ntrA（rpoN）基因的表征及模型推导
Mol Plant Microbe Interact. 1993 Mar-Apr;6(2):238-52. doi: 10.1094/mpmi-6-238.
5
Deletion analysis of the CAP-cAMP binding site of the Escherichia coli lactose promoter.大肠杆菌乳糖启动子CAP-cAMP结合位点的缺失分析
Nucleic Acids Res. 1984 Jul 11;12(13):5449-64. doi: 10.1093/nar/12.13.5449.
6
Use of lac gene fusions in the analysis of Acholeplasma upstream gene regulatory sequences.乳糖操纵子基因融合在无胆甾原体上游基因调控序列分析中的应用。
J Bacteriol. 1994 May;176(9):2763-6. doi: 10.1128/jb.176.9.2763-2766.1994.
7
Construction of a tyrP-lac operon fusion strain and its use in the isolation and analysis of mutants derepressed for tyrP expression.tyrP-乳糖操纵子融合菌株的构建及其在分离和分析tyrP表达去阻遏突变体中的应用。
J Bacteriol. 1984 Oct;160(1):175-83. doi: 10.1128/jb.160.1.175-183.1984.
8
A versatile multiple- and single-copy vector system for the in vitro construction of transcriptional fusions to lacZ.一种用于体外构建与lacZ转录融合体的通用多拷贝和单拷贝载体系统。
Plasmid. 1985 Sep;14(2):134-42. doi: 10.1016/0147-619x(85)90073-3.
9
The -45 region of the Escherichia coli lac promoter: CAP-dependent and CAP-independent transcription.大肠杆菌乳糖启动子的 -45 区域：依赖于代谢物基因激活蛋白（CAP）和不依赖于 CAP 的转录
J Bacteriol. 1997 Jan;179(2):423-9. doi: 10.1128/jb.179.2.423-429.1997.
10
Expression of lacZ from the promoter of the Escherichia coli spc operon cloned into vectors carrying the W205 trp-lac fusion.从克隆到携带W205 trp-lac融合体的载体中的大肠杆菌spc操纵子启动子表达lacZ。
J Bacteriol. 1998 Dec;180(23):6090-100. doi: 10.1128/JB.180.23.6090-6100.1998.
引用本文的文献
1
Pyruvate kinase-deficient Escherichia coli exhibits increased plasmid copy number and cyclic AMP levels.丙酮酸激酶缺陷型大肠杆菌表现出质粒拷贝数增加和环磷酸腺苷水平升高。
J Bacteriol. 2009 May;191(9):3041-9. doi: 10.1128/JB.01422-08. Epub 2009 Feb 27.
2
Efficiency of transcription from promoter sequence variants in Lactobacillus is both strain and context dependent.乳酸杆菌中启动子序列变体的转录效率既取决于菌株，也取决于环境。
J Bacteriol. 1999 Oct;181(20):6569-72. doi: 10.1128/JB.181.20.6569-6572.1999.
3
The effects of mutations in the ant promoter of phage P22 depend on context.噬菌体P22抗启动子突变的影响取决于背景。
Genetics. 1988 Oct;120(2):319-27. doi: 10.1093/genetics/120.2.319.
4
Mutations of the Escherichia coli lacUV5 promoter resulting in increased expression in Bacillus subtilis.大肠杆菌lacUV5启动子的突变导致其在枯草芽孢杆菌中的表达增加。
Mol Gen Genet. 1987 Oct;209(3):467-74. doi: 10.1007/BF00331151.
5
Nucleotide sequence of the kanamycin resistance determinant of the pneumococcal transposon Tn1545: evolutionary relationships and transcriptional analysis of aphA-3 genes.肺炎球菌转座子Tn1545卡那霉素抗性决定簇的核苷酸序列：aphA-3基因的进化关系及转录分析
Mol Gen Genet. 1987 May;207(2-3):509-13. doi: 10.1007/BF00331623.
6
Mutational analysis of the lac regulatory region: second-site changes that activate mutant promoters.乳糖操纵子调控区域的突变分析：激活突变启动子的第二位点变化
Nucleic Acids Res. 1989 May 25;17(10):3909-25. doi: 10.1093/nar/17.10.3909.
7
Mutation of the promoter and LexA binding sites of cea, the gene encoding colicin E1.编码大肠杆菌素E1的cea基因启动子和LexA结合位点的突变
Mol Gen Genet. 1989 Feb;215(3):483-9. doi: 10.1007/BF00427047.
8
Saturation mutagenesis of an Escherichia coli rRNA promoter and initial characterization of promoter variants.大肠杆菌rRNA启动子的饱和诱变及启动子变体的初步表征
J Bacteriol. 1989 Sep;171(9):4852-61. doi: 10.1128/jb.171.9.4852-4861.1989.
9
Hierarchies of base pair preferences in the P22 ant promoter.P22噬菌体抗启动子中碱基对偏好性的层级结构
J Bacteriol. 1991 Mar;173(6):1944-50. doi: 10.1128/jb.173.6.1944-1950.1991.
10
Mutations that affect activity of the Rhizobium meliloti trpE(G) promoter in Rhizobium meliloti and Escherichia coli.影响苜蓿根瘤菌trpE(G)启动子在苜蓿根瘤菌和大肠杆菌中活性的突变。
J Bacteriol. 1991 Sep;173(18):5831-6. doi: 10.1128/jb.173.18.5831-5836.1991.
本文引用的文献
1
Spacer mutations in the lac ps promoter.乳糖操纵子启动子中的间隔突变
Proc Natl Acad Sci U S A. 1982 Feb;79(4):1069-72. doi: 10.1073/pnas.79.4.1069.
2
Mutants of the lac promoter with large insertions and deletions between the CAP binding site and the -35 region.在CAP结合位点与-35区域之间存在大的插入和缺失的乳糖启动子突变体。
Gene. 1984 Nov;31(1-3):263-7. doi: 10.1016/0378-1119(84)90219-1.
3
In vivo expression of lac promoter variants with altered -10, -35, and spacer sequences.具有改变的-10、-35和间隔序列的lac启动子变体的体内表达。
Cold Spring Harb Symp Quant Biol. 1983;47 Pt 1:473-6. doi: 10.1101/sqb.1983.047.01.056.
4
Compilation and analysis of Escherichia coli promoter DNA sequences.大肠杆菌启动子DNA序列的汇编与分析
Nucleic Acids Res. 1983 Apr 25;11(8):2237-55. doi: 10.1093/nar/11.8.2237.
5
The tac promoter: a functional hybrid derived from the trp and lac promoters.Tac启动子：一种源自色氨酸启动子和乳糖启动子的功能性杂种启动子。
Proc Natl Acad Sci U S A. 1983 Jan;80(1):21-5. doi: 10.1073/pnas.80.1.21.
6
New versatile plasmid vectors for expression of hybrid proteins coded by a cloned gene fused to lacZ gene sequences encoding an enzymatically active carboxy-terminal portion of beta-galactosidase.新型多功能质粒载体，用于表达由与编码β-半乳糖苷酶酶活性羧基末端部分的lacZ基因序列融合的克隆基因所编码的杂交蛋白。
Gene. 1983 Nov;25(1):71-82. doi: 10.1016/0378-1119(83)90169-5.
7
Chemical synthesis and biochemical reactivity of bacteriophage lambda PR promoter.噬菌体λ PR 启动子的化学合成与生化反应活性
Nucleic Acids Res. 1983 Feb 11;11(3):773-87. doi: 10.1093/nar/11.3.773.
8
A lac promoter with a changed distance between -10 and -35 regions.一种在-10区和-35区之间距离发生改变的乳糖启动子。
Nucleic Acids Res. 1982 Feb 11;10(3):903-12. doi: 10.1093/nar/10.3.903.
9
E. coli RNA polymerase interacts homologously with two different promoters.大肠杆菌RNA聚合酶与两种不同的启动子进行同源性相互作用。
Cell. 1980 Jun;20(2):269-81. doi: 10.1016/0092-8674(80)90613-3.
10
Sequencing end-labeled DNA with base-specific chemical cleavages.通过碱基特异性化学切割对末端标记的DNA进行测序。
Methods Enzymol. 1980;65(1):499-560. doi: 10.1016/s0076-6879(80)65059-9.
Zotero 插件