乳糖操纵子基因融合在无胆甾原体上游基因调控序列分析中的应用。
Use of lac gene fusions in the analysis of Acholeplasma upstream gene regulatory sequences.
作者信息
Knudtson K L, Minion F C
机构信息
Department of Microbiology, Immunology and Preventive Medicine, Iowa State University, Ames 50011.
出版信息
J Bacteriol. 1994 May;176(9):2763-6. doi: 10.1128/jb.176.9.2763-2766.1994.
Abstract
The objective of this study was to develop a means of identifying and analyzing mycoplasma gene regulatory elements. Analysis of Acholeplasma oculi ISM1499 transcriptional sequences was accomplished using a promoterless lacZ reporter gene and an integrative vector strategy. Seven promoter-containing fragments were identified, levels of beta-galactosidase were determined, and transcriptional start sites were mapped.
摘要
本研究的目的是开发一种鉴定和分析支原体基因调控元件的方法。使用无启动子的lacZ报告基因和整合载体策略完成了对眼无胆甾原体ISM1499转录序列的分析。鉴定出七个含启动子的片段，测定了β-半乳糖苷酶水平，并绘制了转录起始位点。
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