野生型和突变株产生的胞外多糖的表征、抗氧化和免疫调节潜力。
Characterization, antioxidant and immunomodulatory potential on exopolysaccharide produced by wild type and mutant strains.
作者信息
Adebayo-Tayo Bukola, Ishola Racheal, Oyewunmi Titiloye
机构信息
Department of Microbiology, University of Ibadan, Ibadan, Oyo State, Nigeria.
Immunology Unit, Department of Chemical Pathology, College of Medicine, University of Ibadan, Nigeria.
出版信息
Biotechnol Rep (Amst). 2018 Jul 3;19:e00271. doi: 10.1016/j.btre.2018.e00271. eCollection 2018 Sep.
Characterization, antioxidant and immunomodulatory potential of exopolysaccharide (EPS) produced by wild type and mutant was investigated. The EPS production ranged from 5490.2 to 5580.7 mg/L. Wild type (WWCEPS) had the highest EPS production. Eight (8) sugar moieties were present in the EPS. Galactose had the highest EPS composition (34.6 mg/100 g and 33.5 mg/100 g EPS) in Wild type EPS (WWCEPS) and mutant EPS (MWCEPS). Wild type and mutant EPS had antioxidant capacity. The scavenging assay for the antioxidant increased in a dose dependent (0.5-10 mg/mL) manner. Wild type EPS had the highest 1,1-Diphemy 1-2-picryl-hydrazyl (DPPH) capacity, total antioxidant activity, hydrogen peroxide and reducing power activity (71%, 1.9%, 86.7% and 1.9%). The mice injected peritorially with mutant EPS had the highest IgG and IgM (68-87 mg/dL and 64-70 mg/dL). IgA of the mice treated with Wild type EPS increased from 67 to 73 mg/dL. Wild type and mutant EPS had immunomodulatory activity on the treated mice.
研究了野生型和突变体产生的胞外多糖（EPS）的特性、抗氧化和免疫调节潜力。EPS产量范围为5490.2至5580.7毫克/升。野生型（WWCEPS）的EPS产量最高。EPS中存在八种糖部分。在野生型EPS（WWCEPS）和突变体EPS（MWCEPS）中，半乳糖的EPS组成最高（分别为34.6毫克/100克和33.5毫克/100克EPS）。野生型和突变体EPS具有抗氧化能力。抗氧化剂的清除试验以剂量依赖性（0.5 - 10毫克/毫升）方式增加。野生型EPS具有最高的1,1 - 二苯基 - 2 - 苦基肼（DPPH）清除能力、总抗氧化活性、过氧化氢清除能力和还原能力（分别为71%、1.9%、86.7%和1.9%）。腹腔注射突变体EPS的小鼠具有最高的IgG和IgM水平（分别为68 - 87毫克/分升和64 - 70毫克/分升）。用野生型EPS处理的小鼠的IgA从67毫克/分升增加到73毫克/分升。野生型和突变体EPS对处理过的小鼠具有免疫调节活性。
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