University of Edinburgh, Queen's Medical Research Institute, EPSRC IRC Hub in Optical Molecular Sens, United Kingdom.
University of Edinburgh, Institute for Integrated Micro and Nano Systems, School of Engineering, Edi, United Kingdom.
J Biomed Opt. 2018 Jul;23(7):1-12. doi: 10.1117/1.JBO.23.7.076005.
A highly sensitive, modular three-color fluorescence endomicroscopy imaging platform spanning the visible to near-infrared (NIR) range is demonstrated. Light-emitting diodes (LEDs) were sequentially pulsed along with the camera acquisition to provide up to 20 frames per second (fps) three-color imaging performance or 60 fps single color imaging. The system was characterized for bacterial and cellular molecular imaging in ex vivo human lung tissue and for bacterial and indocyanine green imaging in ex vivo perfused sheep lungs. A practical method to reduce background tissue autofluorescence is also proposed. The platform was clinically translated into six patients with pulmonary disease to delineate healthy, cancerous, and fibrotic tissue autofluorescent structures. The instrument is the most broadband clinical endomicroscopy system developed to date (covering visible to the NIR, 500 to 900 nm) and demonstrates significant potential for future clinical utility due to its low cost and modular capability to suit a wide variety of molecular imaging applications.
展示了一种高灵敏度、模块化的三色彩荧光内窥成像平台，涵盖可见到近红外（NIR）范围。发光二极管（LED）与相机采集同步顺序脉冲，可提供高达每秒 20 帧（fps）的三色彩成像性能或 60 fps 的单色彩成像性能。该系统在离体人肺组织中进行了细菌和细胞分子成像以及在离体灌注羊肺中进行了细菌和吲哚菁绿成像的特性进行了研究。还提出了一种实用的方法来减少背景组织自发荧光。该平台已临床转化为 6 例肺部疾病患者，以描绘健康、癌变和纤维化组织的自发荧光结构。该仪器是迄今为止开发的最宽带临床内窥成像系统（覆盖可见到近红外，500 到 900nm），由于其低成本和模块化功能，可适用于各种分子成像应用，因此具有重要的临床应用潜力。
