School of Chemistry and the EPSRC IRC Proteus, University of Edinburgh, Joseph Black Building, David Brewster Road, Edinburgh, EH9 3FJ, UK.
Org Biomol Chem. 2018 Nov 7;16(43):8056-8063. doi: 10.1039/c8ob01790e.
Optical biosensing based on the activation of fluorescent reporters offers a powerful methodology for the real-time molecular interrogation of pathology. Here we report a first-in-class, bimodal fluorescent reporter strategy for the simultaneous and highly specific detection of two independent proteases (thrombin and matrix metalloproteases (MMPs)) pivotal in the fibroproliferative process surrounding lung cancer, based on a dual, multiplexing, peptide FRET system. This sophisticated synthetic smartprobe, with a molecular weight of 6 kDa, contains two independent fluorophores and quenchers that generate photonic signatures at two specific wavelengths upon activation by target enzymes within human lung cancer tissue.
基于荧光报告物激活的光学生物传感为实时分子检测病理学提供了一种强大的方法。在这里，我们报告了一种首创的双模式荧光报告物策略，用于同时高度特异性地检测两种独立的蛋白酶（凝血酶和基质金属蛋白酶（MMPs）），这两种蛋白酶在肺癌周围的纤维增生过程中至关重要，基于双，多重肽 FRET 系统。这种复杂的合成智能探针分子量为 6 kDa，包含两个独立的荧光团和猝灭剂，当在人肺癌组织中的靶酶激活时，会在两个特定波长产生光子特征。
