Parker Helen E, Stone James M, Marshall Adam D L, Choudhary Tushar R, Thomson Robert R, Dhaliwal Kevin, Tanner Michael G
EPSRC Proteus IRC Hub in Optical Molecular Sensing & Imaging, Centre for Inflammation Research, Queen's Medical Research Institute, University of Edinburgh, Edinburgh, UK.
Centre for Photonics and Photonic Materials, Department of Physics, University of Bath, Bath, UK.
Biomed Opt Express. 2019 Mar 15;10(4):1856-1869. doi: 10.1364/BOE.10.001856. eCollection 2019 Apr 1.
Fibre-based optical endomicroscopy (OEM) permits high resolution fluorescence microscopy in endoscopically accessible tissues. Fibred OEM has the potential to visualise pathologies targeted with fluorescent imaging probes and provide an molecular pathology platform to augment disease understanding, diagnosis and stratification. Here we present an inexpensive widefield ratiometric fibred OEM system capable of enhancing the contrast between similar spectra of pathologically relevant fluorescent signals without the burden of complex spectral unmixing. As an exemplar, we demonstrate the potential of the platform to detect fluorescently labelled Gram-negative bacteria in the challenging environment of highly autofluorescent lung tissue in whole human lungs.
基于纤维的光学内镜显微镜（OEM）可在内镜可及的组织中进行高分辨率荧光显微镜检查。纤维OEM有潜力可视化荧光成像探针靶向的病变，并提供一个分子病理学平台，以加深对疾病的理解、诊断和分层。在此，我们展示了一种廉价的宽场比率纤维OEM系统，该系统能够增强病理相关荧光信号相似光谱之间的对比度，而无需复杂的光谱解混负担。作为一个范例，我们证明了该平台在全人类肺脏中高自发荧光肺组织这一具有挑战性的环境中检测荧光标记革兰氏阴性菌的潜力。
