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链霉菌中具有广泛宿主范围的裂解性和溶原性噬菌体的分离与初步鉴定

Isolation and preliminary characterization of lytic and lysogenic phages with wide host range within the streptomycetes.

作者信息

Greene J, Goldberg R B

出版信息

J Gen Microbiol. 1985 Sep;131(9):2459-65. doi: 10.1099/00221287-131-9-2459.

Abstract

Examination of approximately 700 soil samples yielded about 100 actinophages. Restriction analysis of phage DNA indicated that 57 are unique, and of these, 20 produce turbid plaques on one or more of the streptomycetes tested. Five phages are shown to insert into the genome of Streptomyces avermitilis. None of the phages was able to perform generalized transduction of S. avermitilis.

摘要

对大约700个土壤样本进行检测后获得了约100个放线菌噬菌体。噬菌体DNA的限制性分析表明,其中57个是独特的,在这些独特的噬菌体中,有20个在一种或多种受试链霉菌上产生浑浊噬菌斑。有5个噬菌体被证明可插入阿维链霉菌的基因组中。没有一个噬菌体能够对阿维链霉菌进行普遍性转导。

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