冻干和储存温度对班氏吴策线虫抗原敏感性及稳定性的影响。

Effects of lyophilization and storage temperature on Wuchereria bancrofti antigen sensitivity and stability.

作者信息

Agbozo Edem Y, Dumashie Edward, Boakye Daniel A, de Souza Dziedzom K

机构信息

Accra Technical University, Accra, Ghana.

Department of Parasitology, Noguchi Memorial Institute for Medical Research, University of Ghana, Accra, Ghana.

出版信息

BMC Res Notes. 2018 Jul 11;11(1):454. doi: 10.1186/s13104-018-3586-0.

DOI:10.1186/s13104-018-3586-0

PMID:29996898

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6042357/

Abstract

OBJECTIVE

Antigen-based rapid diagnostic tests for Lymphatic filariasis (LF) do not come with external quality control (QC) materials, and research and disease control programmes rely on stored positive samples. This study was undertaken to evaluate the use of lyophilized Wuchereria bancrofti antigen positive plasma samples to serve as QC materials for LF diagnostic tests. 10 well characterized W. bancrofti positive samples were lyophilized and stored at 4, 28 and 40 °C. The samples were evaluated using the Alere Filariasis Test Strips before lyophilization, and after 1 and 3 months of storage. The sensitivity and stability of the lyophilized samples were evaluated.

RESULTS

The results revealed a loss of sensitivity and stability with increasing temperature and duration of storage. The results are further discussed in terms of the use of dried blood spot (DBS) in diagnostic studies on LF, and the need for thoughtful DBS preparation and storage.

摘要

目的

用于淋巴丝虫病（LF）的基于抗原的快速诊断检测不配备外部质量控制（QC）材料，研究和疾病控制项目依赖于储存的阳性样本。本研究旨在评估冻干的班氏吴策线虫抗原阳性血浆样本作为LF诊断检测质量控制材料的用途。对10个特征明确的班氏吴策线虫阳性样本进行冻干，并分别储存在4℃、28℃和40℃。在冻干前以及储存1个月和3个月后，使用Alere丝虫病检测试纸对样本进行评估。评估了冻干样本的敏感性和稳定性。