Department of Biomedical Engineering and Center for Marine-Integrated Biomedical Technology (BK 21 Plus), Pukyong National University, Busan, South Korea.
Interdisciplinary Program of Biomedical Mechanical & Electrical Engineering, Pukyong National University, Busan, South Korea.
Int J Biol Macromol. 2018 Oct 15;118(Pt B):1713-1725. doi: 10.1016/j.ijbiomac.2018.07.018. Epub 2018 Jul 8.
A nanocomposite film, chitosan (CS)-polyvinylpyrrolidone (PVP)-bentonite (BN) was fabricated to enhance wound healing processes as a new nanoplatform for wound dressing. Both physical properties and antibacterial activity of the proposed film were examined to validate its applicability and inhibitory effect for wound management. In vitro cytotoxicity was evaluated by using MTT assay on L929 and NIH3T3 cells to identify the toxicity level of the film. In vivo wound healing test assessed the wound healing performance in animal models. The results confirmed a strong interaction between surface functional groups among CS, PVP and BN with suitable surface morphology and high thermal stability. The CS-PVP-BN film improved various material features such as including mechanical property, tensile strength, pH and porosity, inhibitory activity on bacterial organisms, and collagen deposition. The animal study confirmed that the fabricated film yielded a rapid healing rate of 97%, less scarring, thick granulation at the 11th day, regeneration of epidermis at the 16th day, and abundant deposition of collagen and fibroblast, compared with control. The non-toxic nanocomposite film can be a promising antibacterial wound dressing with rapid wound healing effects in wound care management.
一种纳米复合膜，壳聚糖（CS）-聚乙烯吡咯烷酮（PVP）-膨润土（BN）被制备出来以增强伤口愈合过程，作为伤口敷料的新纳米平台。为了验证其适用性和对伤口管理的抑制作用，对所提出的薄膜的物理性能和抗菌活性进行了检查。通过在 L929 和 NIH3T3 细胞上进行 MTT 测定来评估体外细胞毒性，以确定薄膜的毒性水平。体内伤口愈合试验评估了动物模型中的伤口愈合性能。结果证实了 CS、PVP 和 BN 之间表面官能团之间具有很强的相互作用，具有合适的表面形态和高热稳定性。CS-PVP-BN 薄膜改善了各种材料特性，包括机械性能、拉伸强度、pH 值和孔隙率、对细菌的抑制活性以及胶原蛋白的沉积。动物研究证实，与对照组相比，所制备的薄膜具有 97%的快速愈合率、较少的疤痕、第 11 天有较厚的肉芽组织、第 16 天表皮再生以及丰富的胶原蛋白和纤维母细胞沉积。这种无毒的纳米复合膜有望成为一种具有快速伤口愈合效果的抗菌伤口敷料，可用于伤口护理管理。
