Shi Xiaobin, Chen Gong, Pan Huipeng, Xie Wen, Wu Qingjun, Wang Shaoli, Liu Yong, Zhou Xuguo, Zhang Youjun
Hunan Academy of Agricultural Sciences, Hunan Plant Protection Institute, Changsha, China.
Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, China.
Front Microbiol. 2018 Jun 27;9:1404. doi: 10.3389/fmicb.2018.01404. eCollection 2018.
The sweet potato whitefly, , is one of the most invasive insect pests worldwide. The two most destructive whitefly cryptic species are MEAM1/B and MED/Q. Given that MED/Q has replaced MEAM1/B in China and the invasion of MED/Q has coincided with the outbreak of tomato yellow leaf curl virus (TYLCV), we hypothesize that pre-infestation with viruliferous will affect the subsequent host preferences. To test this hypothesis, we (1) conducted bioassays to compare the host preference of viruliferous and non-viruliferous MEAM1/B and MED/Q, respectively, on plants pre-infested with viruliferous and non-viruliferous MEAM1/B and MED/Q; (2) profiled plant volatiles using GC-MS; and (3) functionally characterized chemical cues could potentially modulate -TYLCV-tomato interactions, including ρ-cymene, thujene and neophytadiene, using a Y-tube olfactometer. As a result, plants pre-infested with MEAM1/B whiteflies carrying TYLCV or not, did not attract more or less B or Q whiteflies. Plants pre-infested with non-viruliferous MED/Q resisted MEAM1/B but did not affect MED/Q. However, plants pre-infested with viruliferous MED/Q attracted more whiteflies. Feeding of viruliferous MED/Q reduced the production of ρ-cymene, and induced thujene and neophytadiene. Functionally analyses of these plant volatiles show that ρ-cymene deters while neophytadiene recruits whiteflies. These combined results suggest that pre-infestation with viruliferous MED/Q promotes the subsequent whitefly infestation and induces plant volatile neophytadiene which recruits whiteflies.
烟粉虱是全球最具入侵性的害虫之一。两种最具破坏性的粉虱隐性物种是MEAM1/B和MED/Q。鉴于MED/Q在中国已取代MEAM1/B，且MED/Q的入侵与番茄黄化曲叶病毒（TYLCV）的爆发同时发生，我们推测用携带病毒的烟粉虱预先侵染会影响后续的寄主偏好。为了验证这一假设，我们（1）进行了生物测定，分别比较携带病毒和不携带病毒的MEAM1/B和MED/Q在预先被携带病毒和不携带病毒的MEAM1/B和MED/Q侵染的植物上的寄主偏好；（2）使用气相色谱-质谱联用仪（GC-MS）分析植物挥发物；（3）使用Y型嗅觉仪对可能调节TYLCV-番茄相互作用的化学信号进行功能表征，包括对伞花烃、侧柏烯和新植二烯。结果表明，预先被携带或未携带TYLCV的MEAM1/B粉虱侵染的植物，吸引的B或Q粉虱数量没有增多或减少。预先被不携带病毒的MED/Q侵染的植物对MEAM1/B有抗性，但对MED/Q没有影响。然而，预先被携带病毒的MED/Q侵染的植物吸引了更多的粉虱。携带病毒的MED/Q取食会减少对伞花烃的产生，并诱导侧柏烯和新植二烯的产生。对这些植物挥发物的功能分析表明，对伞花烃具有驱避作用，而新植二烯能吸引粉虱。这些综合结果表明，预先被携带病毒的MED/Q侵染会促进后续粉虱的侵染，并诱导植物产生能吸引粉虱的挥发物新植二烯。
