Department of Biotechnology, School of Biotechnology, Hochiminh City International University, Vietnam National University-Hochiminh City, Quarter 6, Linh Trung Ward, Thu Duc District, Hochiminh 700000, Vietnam.
Toxins (Basel). 2018 Jul 12;10(7):288. doi: 10.3390/toxins10070288.
Screening for compounds that can neutralize the toxicity of tetrodotoxin (TTX) or reduce its negative effects is necessary. Our study tested the TTX detoxification capacity of exopolysaccharide (EPS) extracted from lactic acid bacteria. EPS of N3 isolated from the Vung Tau sea (Vietnam), PN05, and PN04 were used in the study. To more completely evaluate the importance of EPS in detoxification, EPS samples of N3, PN05 and PN04 were also tested. The majority of EPS of these bacteria contained glucose; this was observed using thin layer chromatography (TLC) and high-performance liquid chromatography (HPLC) analysis. As observed with FTIR analysis, only EPS of PN05 contained methyl groups. The results indicated that detoxification of TTX in mice could be obtained at an optimal dose of 248 µg EPS from incubated with 54 µg cuprous oxide for 40 min or 148 µg EPS incubated with 55 µg cuprous oxide for 40 min, while EPS from showed TTX detoxification capacity without cuprous oxide combination. Consequently, EPS from PN05 can be used in TTX prevention. This is the first report on the importance of lactic acid bacteria in TTX detoxification.
筛选能够中和河豚毒素（TTX）毒性或降低其负面影响的化合物是必要的。我们的研究测试了从乳酸菌中提取的胞外多糖（EPS）对 TTX 的解毒能力。本研究使用了从越南头顿海分离的 N3、PN05 和 PN04 的 EPS。为了更全面地评估 EPS 在解毒中的重要性，还测试了 N3、PN05 和 PN04 的 EPS 样品。这些细菌的大多数 EPS 都含有葡萄糖；这是通过薄层层析（TLC）和高效液相色谱（HPLC）分析观察到的。如 FTIR 分析所示，只有 PN05 的 EPS 含有甲基。结果表明，在最佳剂量下，用 54 µg 氧化亚铜孵育 40 分钟或用 148 µg EPS 孵育 40 分钟，可从 248 µg EPS 中获得对 TTX 的解毒作用，而 则无需与氧化亚铜结合即可显示出 TTX 解毒能力。因此，PN05 的 EPS 可用于 TTX 预防。这是关于乳酸菌在 TTX 解毒中的重要性的首次报道。
