School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing 210094, China.
Biomater Sci. 2018 Jul 24;6(8):2197-2208. doi: 10.1039/c8bm00492g.
Electrospun collagen nanofibers are effective for wound healing; however, many problems, such as the tedious preparation process, weak strength and poor structure integration, limit further applications. In this study, recombinant human collagen (RHC) peptides and a simple one-step crosslinking strategy were used to prepare RHC/chitosan nanofibers. With the nonpathogenic, water-soluble RHC and a mild electrospinning solvent, in situ crosslinked nanofibers (S-CN) not only simplified the preparation procedure but also maintained a more integrated morphology. Compared with the immersed crosslinked nanofibers (I-CN), S-CN showed better performance in moisture retention, degradation and mechanical strength tests. In vitro cell proliferation, morphology and RT-PCR studies confirmed that fibroblasts presented better activities on nanofibers crosslinked in situ. Importantly, after treating with the nanofibers, rapid epidermidalization and angiogenesis were observed in an SD rat scalding model. All these data suggest that electrospun RHC/chitosan nanofibers crosslinked in situ are an ideal candidate that can be used for wound healing applications.
静电纺丝胶原纳米纤维在伤口愈合方面具有很好的效果；但是，许多问题，如繁琐的制备过程、强度较弱和结构集成度差，限制了其进一步的应用。在这项研究中，使用重组人胶原蛋白 (RHC) 肽和简单的一步交联策略来制备 RHC/壳聚糖纳米纤维。使用非致病性、水溶性的 RHC 和温和的静电纺丝溶剂，原位交联纳米纤维（S-CN）不仅简化了制备程序，而且保持了更完整的形态。与浸泡交联纳米纤维（I-CN）相比，S-CN 在保水、降解和机械强度测试方面表现出更好的性能。体外细胞增殖、形态和 RT-PCR 研究证实，成纤维细胞在原位交联的纳米纤维上表现出更好的活性。重要的是，在对 SD 大鼠烫伤模型进行治疗后，观察到表皮快速化和血管生成。所有这些数据表明，静电纺丝 RHC/壳聚糖纳米纤维原位交联是一种理想的候选材料，可用于伤口愈合应用。
