Department of Orthodontics, Shanghai Stomatological Hospital, Fudan University, Shanghai, 200001, China.
J BUON. 2020 Mar-Apr;25(2):1245-1250.
This investigation was undertaken to infer the anticancer effects of rosmarinic acid against human oral cancer cells.
Normal hTRET-OME oral cell line and oral cancer cell line SCC-15 were used in the present study. CDK-8 was used to determine the proliferation of cancer cells. Apoptosis of cancer cells was assessed by DAPI staining method. Flow cytometric procedure was employed to study the cancer cell cycle phase distribution. The migratory potential of cancer cells was estimated by transwell assay.
Rosmarinic acid inhibited the proliferation of oral cancer cells and the level of inhibition was dose-dependent. The antiproliferative role of rosmarinic acid was exerted through apoptotis induction and arrest of cell cycle at G2/M phase in oral cancer cells. Treatment of rosmarinic acid also resulted in endoplasmic reticulum stress and affected negatively the migratory potential of cancer cells in a concentration-dependent manner.
The results of this study revealed the anticancer potential of rosmarinic acid against the oral cancer cell growth and propagation. The study envisages the importance of natural compounds for their usage against human cancers.
本研究旨在推断迷迭香酸对人口腔癌细胞的抗癌作用。
本研究使用正常 hTRET-OME 口腔细胞系和口腔癌细胞系 SCC-15。使用 CDK-8 测定癌细胞的增殖。通过 DAPI 染色法评估癌细胞的凋亡。采用流式细胞术程序研究癌细胞的细胞周期分布。通过 Transwell 测定评估癌细胞的迁移潜力。
迷迭香酸抑制口腔癌细胞的增殖，抑制程度呈剂量依赖性。迷迭香酸通过诱导细胞凋亡和使口腔癌细胞的细胞周期停滞在 G2/M 期发挥抗增殖作用。迷迭香酸处理还导致内质网应激，并以浓度依赖性方式负向影响癌细胞的迁移潜力。
本研究结果揭示了迷迭香酸对口腔癌细胞生长和增殖的抗癌潜力。该研究设想了天然化合物在对抗人类癌症方面的重要性。
