Supino R, Gibelli N, Galatulas I, Zunino F
Cell Biol Int Rep. 1985 Dec;9(12):1059-68. doi: 10.1016/s0309-1651(85)80003-5.
Since the involvement of calcium ions in the regulation of cell division and differentiation has been proposed, in this study we have examined the effect of extracellular calcium and of calcium-modulating agents on the DMSO-induced differentiation of murine erythroleukaemia cells. Neither proliferation nor differentiation of these cells was affected by calcium deprivation in the culture medium. Moreover, calcium-chelating agents or agents blocking intracellular calcium uptake induced a marked inhibition of cell differentiation. Intracellular calcium antagonists induced inhibition when cells were grown in a calcium-deprived medium. In contrast, murine erythroleukaemia cell differentiation was unaffected by agents that increased intracellular concentration of calcium. Our results indicate that a mobilization of calcium is indispensable for eliciting full cellular response, but the increase in intracellular level of this cation is not sufficient for complete signal transduction. It is likely that a marked alteration of the intracellular calcium system and availability could be responsible for the independence of our cell system from calcium modulation.
由于钙离子参与细胞分裂和分化的调节这一观点已被提出，在本研究中，我们检测了细胞外钙和钙调节剂对二甲基亚砜诱导的小鼠红白血病细胞分化的影响。培养基中钙缺乏对这些细胞的增殖和分化均无影响。此外，钙螯合剂或阻断细胞内钙摄取的试剂可显著抑制细胞分化。当细胞在缺钙培养基中生长时，细胞内钙拮抗剂会诱导抑制作用。相反，增加细胞内钙浓度的试剂对小鼠红白血病细胞的分化没有影响。我们的结果表明，钙的动员对于引发完整的细胞反应是必不可少的，但这种阳离子细胞内水平的升高不足以实现完整的信号转导。细胞内钙系统和可用性的显著改变可能是我们的细胞系统不受钙调节影响的原因。
