Department of Physical Chemistry and Electrochemistry, Faculty of Chemistry Jagiellonian University in Krakow, Gronostajowa 2, 30387 Krakow, Poland.
Jerzy Haber Institute of Catalysis and Surface Chemistry, Polish Academy of Sciences, Niezapominajek 8, 30239 Krakow, Poland.
Colloids Surf B Biointerfaces. 2018 Nov 1;171:58-66. doi: 10.1016/j.colsurfb.2018.07.012. Epub 2018 Jul 6.
Nowadays, titanium and its alloys are the most commonly used implantable materials. The surface topography and chemistry of titanium-based implants are responsible for osseointegration. One of the methods to improve biocompatibility of Ti implants is a modification with sodium hydroxide (NaOH) or 3-aminopropyltriethoxysilane (APTES). In the present study, anodic titanium dioxide (ATO) layers were electrochemically fabricated, and then immersed in a NaOH solution or in NaOH and APTES solutions. The functionalized samples were characterized by using scanning electron microscopy (SEM) and X-ray photoelectron spectroscopy (XPS). All samples were examined as drug delivery systems and scaffolds for cell culturing. Based on the parameters of the fitted desorption-desorption-diffusion (DDD) model parameters, it was concluded that the modification with NaOH increased the amount of released ibuprofen and inhibited the release process. Osteoblast-like cell line (SAOS-2) was used to investigate the cell response on the non-modified and modified ATO samples. The MTS test and immunofluorescent staining were carried out to examine cell adhesion and proliferation. The data showed that the modification of nanoporous TiO layers with small molecules such as APTES enhanced metabolic activity of adhered cells compared with the non-modified and NaOH-modified TiO layers. In addition, the cells had a polygonal-like morphology with distinct projecting actin filaments and were well dispersed over the whole analyzed surface.
如今，钛及其合金是最常用的植入材料。钛基植入物的表面形貌和化学性质决定了其骨整合能力。提高 Ti 植入物生物相容性的方法之一是用氢氧化钠（NaOH）或 3-氨丙基三乙氧基硅烷（APTES）进行改性。在本研究中，通过电化学方法制备了阳极氧化的二氧化钛（ATO）层，然后将其浸入 NaOH 溶液或 NaOH 和 APTES 溶液中。通过扫描电子显微镜（SEM）和 X 射线光电子能谱（XPS）对功能化样品进行了表征。所有样品均被用作药物输送系统和细胞培养支架进行了研究。基于拟合的解吸-扩散-扩散（DDD）模型参数，可以得出结论，NaOH 改性增加了布洛芬的释放量并抑制了释放过程。成骨样细胞系（SAOS-2）用于研究非改性和改性 ATO 样品上的细胞反应。通过 MTS 测试和免疫荧光染色来检查细胞黏附和增殖情况。结果表明，与未改性和 NaOH 改性的 TiO 层相比，小分子如 APTES 修饰的纳米多孔 TiO 层增强了黏附细胞的代谢活性。此外，细胞呈多边形形态，带有明显突出的肌动蛋白丝，并且在整个分析表面上分布良好。
