Pawlik Anna, Jarosz Magdalena, Syrek Karolina, Sulka Grzegorz D
Department of Physical Chemistry and Electrochemistry, Faculty of Chemistry, Jagiellonian University in Krakow, Ingardena 3, 30060 Krakow, Poland.
Department of Physical Chemistry and Electrochemistry, Faculty of Chemistry, Jagiellonian University in Krakow, Ingardena 3, 30060 Krakow, Poland.
Colloids Surf B Biointerfaces. 2017 Apr 1;152:95-102. doi: 10.1016/j.colsurfb.2017.01.011. Epub 2017 Jan 8.
Although single-drug therapy may prove insufficient in treating bacterial infections or inflammation after orthopaedic surgeries, complex therapy (using both an antibiotic and an anti-inflammatory drug) is thought to address the problem. Among drug delivery systems (DDSs) with prolonged drug release profiles, nanoporous anodic titanium dioxide (ATO) layers on Ti foil are very promising. In the discussed research, ATO samples were synthesized via a three-step anodization process in an ethylene glycol-based electrolyte with fluoride ions. The third step lasted 2, 5 and 10min in order to obtain different thicknesses of nanoporous layers. Annealing the as-prepared amorphous layers at the temperature of 400°C led to obtaining the anatase phase. In this study, water-insoluble ibuprofen and water-soluble gentamicin were used as model drugs. Three different drug loading procedures were applied. The desorption-desorption-diffusion (DDD) model of the drug release was fitted to the experimental data. The effects of crystalline structure, depth of TiO nanopores and loading procedure on the drug release profiles were examined. The duration of the drug release process can be easily altered by changing the drug loading sequence. Water-soluble gentamicin is released for a long period of time if gentamicin is loaded in ATO as the first drug. Additionally, deeper nanopores and anatase phase suppress the initial burst release of drugs. These results confirm that factors such as morphological and crystalline structure of ATO layers, and the procedure of drug loading inside nanopores, allow to alter the drug release performance of nanoporous ATO layers.
尽管单药治疗在治疗骨科手术后的细菌感染或炎症方面可能被证明是不够的，但联合治疗（同时使用抗生素和抗炎药）被认为可以解决这个问题。在具有延长药物释放曲线的药物递送系统（DDS）中，钛箔上的纳米多孔阳极二氧化钛（ATO）层非常有前景。在本研究中，ATO样品是通过在含氟离子的乙二醇基电解质中进行三步阳极氧化过程合成的。第三步持续2、5和10分钟，以获得不同厚度的纳米多孔层。在400°C的温度下对制备好的非晶层进行退火处理，得到锐钛矿相。在本研究中，水不溶性布洛芬和水溶性庆大霉素被用作模型药物。应用了三种不同的药物负载程序。将药物释放的解吸-解吸-扩散（DDD）模型拟合到实验数据中。研究了晶体结构、TiO纳米孔深度和负载程序对药物释放曲线的影响。通过改变药物负载顺序可以很容易地改变药物释放过程的持续时间。如果将庆大霉素作为第一种药物负载到ATO中，水溶性庆大霉素会长时间释放。此外，更深的纳米孔和锐钛矿相会抑制药物的初始突释。这些结果证实，诸如ATO层的形态和晶体结构以及纳米孔内药物负载程序等因素，可以改变纳米多孔ATO层的药物释放性能。
