Departamento de Microbiología e Inmunología, Universidad Nacional de Río Cuarto, Ruta 36 km. 601. (5800), Río Cuarto, Córdoba, Argentina; Member of Consejo Nacional de Investigaciones Científicas y Tecnológicas (CIC-CONICET), Argentina.
Departamento de Microbiología e Inmunología, Universidad Nacional de Río Cuarto, Ruta 36 km. 601. (5800), Río Cuarto, Córdoba, Argentina.
Food Res Int. 2018 Sep;111:306-313. doi: 10.1016/j.foodres.2018.05.026. Epub 2018 May 17.
The objectives of this study were: to evaluate the use of dry distillery grain soluble extract - DDGse to produce yeast biomass and to obtain cell wall (CW), to use the CW as an aflatoxin B (AFB) adsorbent, to study the variation in the composition and thickness of the CW under the influence of DDGse to evaluate their implication on the adsorption process using transmission electron microscopy (TEM) and fourier-transform infrared spectroscopy (FITR). The production of biomass and CW were variable. The CW thickness values showed that S. boulardii strain grown in yeast extract peptone dextrose (YPD) or DDGse medium, with no significant differences observed. The thickness of the CW for S. cerevisiae (RC012 and VM014) were increased when the cells were grown in DDGse medium, the thickness was almost double compared to the values obtained in YPD medium. The spectra IR of each CW in the two culture media shown regions corresponding to polysaccharides, proteins and lipids. Cells grown in DDGse medium adsorbed more AFB than those grown in YPD. The CW adsorbed more AFB than the same amount of whole cell. Future studies should be done to determine the type of carbohydrates and the relationship between chitin - beta glucans responsible for mycotoxin adsorption.
评估利用酒糟可溶物提取物(DDGse)生产酵母生物质和获得细胞壁(CW),并将 CW 用作黄曲霉毒素 B(AFB)的吸附剂,利用透射电子显微镜(TEM)和傅里叶变换红外光谱(FITR)研究 CW 成分和厚度在 DDGse 影响下的变化,评估其对吸附过程的影响。生物质和 CW 的产量是可变的。CW 厚度值表明,在酵母提取物-胰蛋白胨-葡萄糖(YPD)或 DDGse 培养基中生长的 S. boulardii 菌株,没有观察到显著差异。当细胞在 DDGse 培养基中生长时,S. cerevisiae(RC012 和 VM014)的 CW 厚度增加,与在 YPD 培养基中获得的值相比,厚度几乎增加了一倍。在两种培养基中,每个 CW 的红外光谱显示出对应于多糖、蛋白质和脂质的区域。在 DDGse 培养基中生长的细胞吸附的 AFB 多于在 YPD 培养基中生长的细胞。与等量的完整细胞相比,CW 吸附了更多的 AFB。未来的研究应确定负责吸附真菌毒素的碳水化合物类型和几丁质-β葡聚糖之间的关系。