Pereyra Carina, Del Pilar Monge María, Bongiovanni Silvestre, Cristofolini Andrea, Campos Sergio, Cavaglieri Lilia
Departamento de Microbiología e Inmunología, Universidad Nacional de Río Cuarto, Ruta 36, Km 601, 5800 Río Cuarto, Córdoba, Argentina; Instituto Para el Desarrollo Agroindustrial y de la Salud (IDAS), Member of Consejo Nacional de Investigaciones Científicas y Tecnológicas (CIC-CONICET), Argentina.
Departamento de Química, Universidad Nacional de Río Cuarto, Ruta 36, Km 601, 5800 Río Cuarto, Córdoba, Argentina; Member of Consejo Nacional de Investigaciones Científicas y Tecnológicas (CIC-CONICET), Argentina.
Rev Argent Microbiol. 2025 Apr-Jun;57(2):89-97. doi: 10.1016/j.ram.2024.07.004. Epub 2024 Sep 20.
The aim of this study was to determine the impact of Kluyveromyces marxianus VM004 culture conditions on the cell wall (CW) structure and its influence on aflatoxin B binding. The yeast was inoculated into two types of culture media: yeast extract-peptone-dextrose (YPD) broth and dried distiller's grains with solubles (DDG). The CW was extracted from the biomass produced in these media. AFB (150ng/ml) adsorption tests using the biomass (1×10cells/ml) and the CW (0.001g) were performed at pH 2 and pH 8. Transmission electron microscopy (TEM) evaluated the CW thickness, and infrared spectroscopy (IR) determined the CW composition. Biomass production in YPD was higher than that in DDG. Cell diameter (μm) and CW thickness (μm) increased in the DDG medium. The CW percentage obtained in DDG was higher than that in YPD. The absorbance of carbohydrates by IR was higher in YPD. pH influenced AFB adsorption, which was lower at pH 8. The proportion of β-glucan and chitin present in CW was higher in the YPD medium. The IR method allowed to study the CW carbohydrate variation under the influence of these carbon sources. In conclusion, the culture media composition influenced the β-glucan and chitin composition and consequently, AFB adsorption.
本研究的目的是确定马克斯克鲁维酵母VM004培养条件对细胞壁（CW）结构的影响及其对黄曲霉毒素B结合的影响。将酵母接种到两种类型的培养基中：酵母提取物-蛋白胨-葡萄糖（YPD）肉汤和含可溶物的干酒糟（DDG）。从在这些培养基中产生的生物质中提取细胞壁。使用生物质（1×10个细胞/毫升）和细胞壁（0.001克）在pH 2和pH 8下进行黄曲霉毒素B（150纳克/毫升）吸附试验。透射电子显微镜（TEM）评估细胞壁厚度，红外光谱（IR）确定细胞壁组成。YPD中的生物质产量高于DDG中的。DDG培养基中细胞直径（微米）和细胞壁厚度（微米）增加。DDG中获得的细胞壁百分比高于YPD中的。YPD中通过红外光谱测得的碳水化合物吸光度更高。pH影响黄曲霉毒素B的吸附，在pH 8时吸附较低。YPD培养基中细胞壁中β-葡聚糖和几丁质的比例更高。红外光谱法能够研究在这些碳源影响下细胞壁碳水化合物的变化。总之，培养基组成影响β-葡聚糖和几丁质组成，进而影响黄曲霉毒素B的吸附。
