Department of Preventive Veterinary Medicine, Federal University of Santa Maria, UFSM, Santa Maria, RS, 97105-900, Brazil.
Department of Civil and Environmental, Universidad de La Costa, CUC, Calle 58 # 55-66, Barranquilla, Atlántico, Colombia.
Mycotoxin Res. 2024 Feb;40(1):111-121. doi: 10.1007/s12550-023-00508-z. Epub 2023 Nov 14.
The present study aims to evaluate and compare antimycotoxin additives (AMAs) composed of bentonite (AMA 1), clinoptilolite (AMA 2), and beta-glucans extracted from yeast cell wall (AMA 3), with respect to their ability to bind Aflatoxin B (AFB) using the isothermal models of Freundlich, Langmuir, and BET. The additives were submitted to an in vitro adsorption experiment with AFB (0.05-4 mg L), using solutions of pH 3 and pH 6, with an inclusion rate of 0.5%, and analyzed by HPLC-MS/MS. At pH 3, for the seven concentrations evaluated, AMA 1 obtained adsorption rates (99.69 to 99.98%) higher (p < 0. 05) than the other AMAs, which were from 82.97 to 88.72% (AMA 2) and from 79.43 to 89.32% (AMA 3). At pH 6, in concentrations of 1, 2, and 4 mg L of AFB, AMA 1 obtained higher (p < 0.05) adsorption results (97.86 to 99.86%) than AMA 2 (91.98 to 96.12%) and AMA 3 (87.56 to 93.50%). The Freundlich model best fitted the AMA 1 adsorption data. For the other additives, the Langmuir model obtained the best fit, demonstrating q of 8.6 mg g at pH 3 and 2.3 mg g at pH 6 for AMA 2; and for AMA 3, with q of 3.4 mg g at pH 3 and 2.3 mg g at pH 6. The isotherm models work as an effective tool to describe the adsorption process whereas the AMA adsorption capacity varies as a function of product composition, pH, and mycotoxin content.
本研究旨在评估和比较由膨润土（AMA1）、斜发沸石（AMA2）和酵母细胞壁提取的β-葡聚糖组成的抗真菌毒素添加剂（AMA），就其结合黄曲霉毒素 B（AFB）的能力而言，使用弗伦德利希（Freundlich）、朗缪尔（Langmuir）和 BET 等温模型。添加剂在 pH 3 和 pH 6 的溶液中与 AFB（0.05-4 mg L）进行体外吸附实验，包含率为 0.5%，并用 HPLC-MS/MS 进行分析。在 pH 3 下，对于评价的七种浓度，AMA1 的吸附率（99.69 至 99.98%）更高（p<0.05），而其他 AMAs 的吸附率为 82.97 至 88.72%（AMA2）和 79.43 至 89.32%（AMA3）。在 pH 6 下，在 AFB 的 1、2 和 4 mg L 浓度下，AMA1 的吸附结果（97.86 至 99.86%）更高（p<0.05），而 AMA2（91.98 至 96.12%）和 AMA3（87.56 至 93.50%）。Freundlich 模型最适合 AMA1 的吸附数据。对于其他添加剂，Langmuir 模型获得了最佳拟合，表明 pH 3 下的 q 为 8.6 mg g，pH 6 下的 q 为 2.3 mg g，对于 AMA2；对于 AMA3，在 pH 3 下的 q 为 3.4 mg g，在 pH 6 下的 q 为 2.3 mg g。等温线模型是描述吸附过程的有效工具，而 AMA 的吸附能力随产品组成、pH 和真菌毒素含量的变化而变化。
