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癌胚抗原相关细胞黏附分子1（CEACAM-1）基因敲低对人结肠癌细胞的影响。

Effect of CEACAM-1 knockdown in human colorectal cancer cells.

作者信息

Han Zhong-Min, Huang He-Mei, Sun Yong-Wu

机构信息

Department of Medical Technology, Zhengzhou Railway Vocational and Technical College, Zhengzhou, Henan 450052, P.R. China.

出版信息

Oncol Lett. 2018 Aug;16(2):1622-1626. doi: 10.3892/ol.2018.8835. Epub 2018 May 30.

DOI:10.3892/ol.2018.8835
PMID:30008845
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6036324/
Abstract

Carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM-1) is the major antigen of the CD66 cluster of granulocyte differentiation antigens. The present study aimed to assess the biological function of CEACAM-1 on the growth of human colorectal cancer (CRC) cells . Treatment of cultured CRC HCT-8 cells with CEACAM-1-specific siRNA successfully downregulated CEACAM-1 expression by 61% compared with control cells. The effects of CEACAM-1 downregulation on HCT-8 cell proliferation and apoptosis were then assessed via Cell Counting kit-8 assay and flow cytometry, respectively. The results demonstrated that siRNA-induced CEACAM-1 downregulation significantly inhibited proliferation and increased apoptosis, but had no significant effect on cell cycle progression in HCT-8 cells. Together, these results suggest that CEACAM-1 activity is critical to CRC growth, and thus, CEACAM-1 may be a promising therapeutic target for the treatment of CRC.

摘要

癌胚抗原相关细胞粘附分子1（CEACAM-1）是粒细胞分化抗原CD66簇的主要抗原。本研究旨在评估CEACAM-1对人结肠直肠癌（CRC）细胞生长的生物学功能。用CEACAM-1特异性siRNA处理培养的CRC HCT-8细胞，与对照细胞相比，成功将CEACAM-1表达下调了61%。然后分别通过细胞计数试剂盒-8检测和流式细胞术评估CEACAM-1下调对HCT-8细胞增殖和凋亡的影响。结果表明，siRNA诱导的CEACAM-1下调显著抑制增殖并增加凋亡，但对HCT-8细胞的细胞周期进程没有显著影响。总之，这些结果表明CEACAM-1活性对CRC生长至关重要，因此，CEACAM-1可能是治疗CRC的一个有前景的治疗靶点。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef1e/6036324/e6cc218e3c29/ol-16-02-1622-g03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef1e/6036324/3bc8067d9ea5/ol-16-02-1622-g00.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef1e/6036324/4678ed6ae1ef/ol-16-02-1622-g01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef1e/6036324/669e1deff015/ol-16-02-1622-g02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef1e/6036324/e6cc218e3c29/ol-16-02-1622-g03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef1e/6036324/3bc8067d9ea5/ol-16-02-1622-g00.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef1e/6036324/4678ed6ae1ef/ol-16-02-1622-g01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef1e/6036324/669e1deff015/ol-16-02-1622-g02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef1e/6036324/e6cc218e3c29/ol-16-02-1622-g03.jpg

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