脑膜整装标本制备及通过流式细胞术对神经细胞进行表征。
Meningeal whole mount preparation and characterization of neural cells by flow cytometry.
作者信息
Louveau Antoine, Filiano Anthony J, Kipnis Jonathan
机构信息
Center for Brain Immunology and Glia, School of Medicine, University of Virginia, Charlottesville, Virginia.
Department of Neuroscience, School of Medicine, University of Virginia, Charlottesville, Virginia.
出版信息
Curr Protoc Immunol. 2018 Apr;121(1):e50. doi: 10.1002/cpim.50. Epub 2018 Apr 16.
Abstract
Neuroimmunologists aim to understand the interactions between the central nervous system and the immune system under both homeostatic and pathological conditions. The meninges, contrary to the brain parenchyma, are populated by numerous immune cells. Soluble factors produced by these cells are capable to diffuse into the brain parenchyma and influence the brain cells within the parenchyma, including neurons. In this unit, we will describe two protocols: analysis the meningeal compartment of rodents and the use flow cytometry to study the cells of the brain parenchyma (particularly neurons).
摘要
神经免疫学家旨在了解在稳态和病理条件下中枢神经系统与免疫系统之间的相互作用。与脑实质不同，脑膜中有大量免疫细胞。这些细胞产生的可溶性因子能够扩散到脑实质中，并影响实质内的脑细胞，包括神经元。在本单元中，我们将描述两种方案：分析啮齿动物的脑膜腔室以及使用流式细胞术研究脑实质细胞（特别是神经元）。
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