Giani C, D'Amore E, Delarue J C, Mouriesse H, May-Levin F, Sancho-Garnier H, Breccia M, Contesso G
Int J Cancer. 1986 Jan 15;37(1):7-10. doi: 10.1002/ijc.2910370103.
The pattern of estrogen (ER) and progesterone receptors (PR) and their relationship to histo- and cyto-pathological parameters has been studied in 97 cases of benign breast disease and benign phyllode tumors (95 women, of whom 76 were premenopausal, and 2 men). Total (cytosolic + nuclear) ER and PR were assayed by a single-saturating dose method using a tris-KCl buffer. The cut-off between positive and negative ER and PR assay was 100 femtomoles/g tissue. All specimens were processed for histological examination: epithelial and fibroblastic proliferation, epithelial/stromal ratio and presence of focal or diffuse hyalinosis. In 33% of the 46 cases of fibrocystic disease one receptor at least was present (13% ER+, 31% PR+). All the 8 cases in which infiltrating epitheliosis was present were PR+ and 4 of them were also ER+. In 72% of the 31 fibroadenomas one receptor at least was present (19% ER+, 71% PR+). In all these cases levels of receptors were lower than in malignant tumors. An inverse correlation between PR + prevalence and fibrohyalinosis was observed; on the other hand a positive relationship between PR + and fibroblastic (p less than 0.001) or epithelial (p less than 0.01) proliferation was found. In all 5 benign phyllode tumors examined PR + were present at a very high level, almost as high as in malignant tumors. Of the 15 other benign breast lesions, all but one (1 hamartoma) were ER- and PR-.
对97例乳腺良性疾病和良性叶状肿瘤患者(95名女性,其中76名处于绝经前,2名男性)的雌激素(ER)和孕激素受体(PR)模式及其与组织病理学和细胞病理学参数的关系进行了研究。采用单一饱和剂量法,使用三羟甲基氨基甲烷 - 氯化钾缓冲液测定总(胞质 + 核)ER和PR。ER和PR检测阳性与阴性的临界值为100飞摩尔/克组织。所有标本均进行组织学检查:上皮和纤维母细胞增殖、上皮/间质比以及局灶性或弥漫性玻璃样变的存在情况。在46例纤维囊性疾病中,33%至少存在一种受体(13% ER阳性,31% PR阳性)。所有8例存在浸润性上皮增生的病例均为PR阳性,其中4例也为ER阳性。在31例纤维腺瘤中,72%至少存在一种受体(19% ER阳性,71% PR阳性)。在所有这些病例中,受体水平均低于恶性肿瘤。观察到PR阳性患病率与纤维玻璃样变之间呈负相关;另一方面,发现PR阳性与纤维母细胞(p < 0.001)或上皮(p < 0.01)增殖之间呈正相关。在所有检查的5例良性叶状肿瘤中,PR阳性水平非常高,几乎与恶性肿瘤一样高。在其他15例乳腺良性病变中,除1例(1例错构瘤)外,均为ER阴性和PR阴性。