胡宁病毒诱导豚鼠染色体畸变。减毒株XJ克隆3与咖啡因之间的协同作用。

Junin virus-induced chromosomal aberrations in the guinea pig. Synergism between the attenuated strain XJ-clone 3 and caffeine.

作者信息

Dulout F N, Panisse H E, Carballal G, von Guradze H N, De Luca J C, Oubiña J R, Videla C

出版信息

Intervirology. 1985;24(4):193-8. doi: 10.1159/000149643.

Abstract

The frequency of chromosomal aberrations in bone marrow cells of guinea pigs inoculated with the pathogenic XJ strain of Junin virus increased significantly at 6, 9, and 11 days postinoculation (p.i.). Animals inoculated with the attenuated XJ-clone 3 strain only showed significant increments of achromatic lesions (gaps) at 9 days p.i. Guinea pigs inoculated with the XJ-clone 3 strain and then treated with two doses of caffeine 24 and 12 h before killing at 9 days p.i. exhibited a significant increase of chromatid breaks and a parallel decrease of gaps. Because caffeine acts as an inhibitor of repair mechanisms of genetic damage, these results suggest a mutagenic effect of the attenuated strain.

摘要

接种胡宁病毒致病性XJ株的豚鼠骨髓细胞中染色体畸变频率在接种后6天、9天和11天显著增加。接种减毒XJ克隆3株的动物仅在接种后9天出现无色病变（间隙）显著增加。在接种后9天处死前24小时和12小时用两剂咖啡因处理的接种XJ克隆3株的豚鼠，其染色单体断裂显著增加，间隙平行减少。由于咖啡因作为遗传损伤修复机制的抑制剂，这些结果提示减毒株具有诱变作用。