Jining Medical University, Jining, Shandong 272067, China.
Collaborative Innovation Center for Birth Defect Research and Transformation of Shandong Province, Jining Medical University, Jining, Shandong 272067, China.
Biomed Res Int. 2018 Jun 19;2018:6242173. doi: 10.1155/2018/6242173. eCollection 2018.
High-risk HPV is clearly associated with cervical cancer. HPV integration has been confirmed to promote carcinogenesis in the previous studies. In our study, a total of 285 DNA breakpoints and 287 RNA breakpoints were collected. We analyzed the characteristic of HPV integration in the DNA and RNA samples. The results revealed that the patterns of HPV integration in RNA and DNA samples differ significantly. , , and were the hotspot genes integrated by HPV in the DNA samples. , , , , , , , and were the hotspot genes integrated by HPV in RNA samples. Breakpoints of DNA samples were significantly prone to the region of INTRON (P < 0.01, Chi-squared test), whereas in the RNA samples, the breakpoints were prone to EXON. Pathway analysis had revealed that the breakpoints of RNA samples were enriched in the pathways of transcriptional misregulation in cancer, cancer pathway, and pathway of adherens junction. Breakpoints of DNA samples were enriched in the pathway of cholinergic synapse. In summary, our data helped to gain insights into the HPV integration sites in DNA and RNA samples of cervical cancer. It had provided theoretical basis for understanding the mechanism of tumorigenesis from the perspective of HPV integration in the HPV-associated cervical cancers.
高危型人乳头瘤病毒（HPV）与宫颈癌明确相关。HPV 整合已被先前的研究证实可促进致癌作用。在我们的研究中，共收集了 285 个 DNA 断点和 287 个 RNA 断点。我们分析了 HPV 在 DNA 和 RNA 样本中的整合特征。结果表明，HPV 在 RNA 和 DNA 样本中的整合模式存在显著差异。在 DNA 样本中，HPV 整合的热点基因为 、 、 。在 RNA 样本中，HPV 整合的热点基因为 、 、 、 、 、 、 。DNA 样本的断点明显倾向于 INTRON 区域（P < 0.01，卡方检验），而在 RNA 样本中，断点倾向于 EXON。通路分析显示，RNA 样本的断点在癌症转录调控异常、癌症通路和黏着连接通路中富集。DNA 样本的断点在胆碱能突触通路中富集。总之，我们的数据有助于深入了解宫颈癌中 HPV 在 DNA 和 RNA 样本中的整合位点。这为从 HPV 相关宫颈癌 HPV 整合的角度理解肿瘤发生机制提供了理论依据。
