Zhao Yanteng, Zhang Qiang, Zhao Lei, Gan Li, Yi Li, Zhao Yanan, Xue Jingling, Luo Lihua, Du Qiaoyue, Geng Rongxin, Sun Zhihong, Benkirane-Jessel Nadia, Chen Pu, Li Yinping, Chen Yun
Department of Biomedical Engineering, School of Basic Medical Sciences, Wuhan University, Wuhan 430071, China.
Department of Transfusion, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China.
ACS Omega. 2017 Nov 30;2(11):7471-7481. doi: 10.1021/acsomega.7b01003. Epub 2017 Nov 1.
Multichannel nerve guide conduits (MCNGCs) have been widely studied and exhibited outstanding nerve repair function. However, the effect of the geometric structure of MCNGCs on the nerve repair function was still not clear. Herein, we postulated that MCNGCs with different inner surface area-to-volume ratios (ISA/V) of the channels inside the nerve guide conduits (NGCs) would show different nerve repair functions. Therefore, in current work, we constructed a series of hydroxyethyl cellulose/soy protein sponge-based nerve conduit (HSSN) with low, medium, and high ISA/V from hydroxyethyl cellulose (HEC)/soy protein isolate (SPI) composite sponges, which were abbreviated as HSSN-L, HSSN-M and HSSN-H, respectively. These NGCs were applied to bridge and repair a 10 mm long sciatic nerve defect in a rat model. Finally, the influence of ISA/V on nerve repair function was evaluated by electrophysiological assessment, histological investigation, and in vivo biodegradability testing. The results of electrophysiological assessment and histological investigation showed that the regenerative nerve tissues bridged with HSSN-H and HSSN-M had higher compound muscle action potential amplitude ratio, higher percentage of positive NF200 and S100 staining, larger axon diameter, lower -ratio, and greater myelination thickness. Furthermore, the regenerative nerve tissues bridged with HSSN-H also showed higher density of regenerated myelinated nerve fibers and more number of myelin sheath layers. On the whole, the repair efficiency of the peripheral nerve in HSSN-H and HSSN-M groups might be better than that in HSSN-L. These results indicated that higher ISA/V based on HEC/SPI composite sponge may result in greater nerve repair functions. The conclusion provided a probable guiding principle for the structural designs of NGCs in the future.
多通道神经导管（MCNGCs）已得到广泛研究，并表现出出色的神经修复功能。然而，MCNGCs的几何结构对神经修复功能的影响仍不明确。在此，我们推测神经导管（NGCs）内部通道具有不同内表面积与体积比（ISA/V）的MCNGCs会表现出不同的神经修复功能。因此，在当前工作中，我们用羟乙基纤维素（HEC）/大豆分离蛋白（SPI）复合海绵构建了一系列具有低、中、高ISA/V的基于羟乙基纤维素/大豆蛋白海绵的神经导管（HSSN），分别简称为HSSN-L、HSSN-M和HSSN-H。将这些NGCs应用于大鼠模型中桥接和修复10毫米长的坐骨神经缺损。最后，通过电生理评估、组织学研究和体内生物降解性测试评估ISA/V对神经修复功能的影响。电生理评估和组织学研究结果表明，与HSSN-H和HSSN-M桥接的再生神经组织具有更高的复合肌肉动作电位振幅比、更高的NF200和S100阳性染色百分比、更大的轴突直径、更低的比率以及更大的髓鞘厚度。此外，与HSSN-H桥接的再生神经组织还显示出更高密度的再生有髓神经纤维和更多的髓鞘层数。总体而言，HSSN-H和HSSN-M组周围神经的修复效率可能优于HSSN-L组。这些结果表明，基于HEC/SPI复合海绵的更高ISA/V可能导致更强的神经修复功能。该结论为未来NGCs结构设计提供了一条可能的指导原则。
