Dahl J S, Dahl C E
Biochem Biophys Res Commun. 1985 Dec 31;133(3):844-50. doi: 10.1016/0006-291x(85)91211-2.
The effect of ergosterol on cell division and phospholipid metabolism was investigated in Saccharomyces cerevisiae strain GL7, a sterol and unsaturated fatty acid auxotroph. Cells growing poorly on cholesterol were stimulated to grow more rapidly by supplementing the medium with 100 ng of ergosterol per ml. Within 10 min after ergosterol addition to cells prelabeled with 32Pi or [3H]inositol the isotope content of the polyphosphoinositides increases markedly followed by an equally striking and rapid decrease. Subsequently upon continuous labeling, 32P incorporation into phosphatidylinositol and, to a lesser degree, other phospholipids increased. Finally 3h after ergosterol addition the growth rate increased. Only stimulation of the first process, i.e. polyphosphoinositide metabolism, upon ergosterol addition is resistant to inhibition by cycloheximide.
在酿酒酵母菌株GL7（一种甾醇和不饱和脂肪酸营养缺陷型菌株）中研究了麦角固醇对细胞分裂和磷脂代谢的影响。在以胆固醇为生长底物时生长不良的细胞，通过在培养基中添加每毫升100纳克麦角固醇，可刺激其生长得更快。在用³²Pi或[³H]肌醇预标记的细胞中添加麦角固醇后10分钟内，多磷酸肌醇的同位素含量显著增加，随后同样显著且迅速下降。随后在持续标记过程中，³²P掺入磷脂酰肌醇以及在较小程度上掺入其他磷脂的量增加。最后，添加麦角固醇3小时后生长速率增加。麦角固醇添加后仅对第一个过程（即多磷酸肌醇代谢）的刺激对环己酰亚胺的抑制具有抗性。
