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用于同时检测水中三种藻毒素的新型微流控分析传感平台

Novel Microfluidic Analytical Sensing Platform for the Simultaneous Detection of Three Algal Toxins in Water.

作者信息

Maguire Ivan, Fitzgerald Jenny, Heery Brendan, Nwankire Charles, O'Kennedy Richard, Ducrée Jens, Regan Fiona

机构信息

School of Chemical Sciences, DCU Water Institute, School of Biotechnology, School of Physical Sciences, Dublin City University, Glasnevin, Dublin 9, Ireland.

出版信息

ACS Omega. 2018 Jun 30;3(6):6624-6634. doi: 10.1021/acsomega.8b00240. Epub 2018 Jun 20.

DOI:10.1021/acsomega.8b00240
PMID:30023955
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6045346/
Abstract

Globally, the need for "on-site" algal-toxin monitoring has become increasingly urgent due to the amplified demand for fresh-water and for safe, "toxin-free" shellfish and fish stocks. Herein, we describe the first reported, Lab-On-A-Disc (LOAD) based-platform developed to detect microcystin levels in situ, with initial detectability of saxitoxin and domoic acid also reported. Using recombinant antibody technology, the LOAD platform combines immunofluorescence with centrifugally driven microfluidic liquid handling to achieve a next-generation disposable device capable of multianalyte sampling. A low-complexity "LED-photodiode" based optical sensing system was tailor-made for the platform, which allows the fluorescence signal of the toxin-specific reaction to be quantified. This system can rapidly and accurately detect the presence of microcystin-LR, domoic acid, and saxitoxin in 30 min, with a minimum of less than 5 min end-user interaction for maximum reproducibility. This method provides a robust "point of need" diagnostic alternative to the current laborious and costly methods used for qualitative toxin monitoring.

摘要

在全球范围内，由于对淡水以及安全的“无毒素”贝类和鱼类种群的需求不断增加，“现场”藻类毒素监测的需求变得越来越迫切。在此，我们描述了首个报道的基于盘上实验室（LOAD）的平台，该平台用于原位检测微囊藻毒素水平，同时也报道了其对石房蛤毒素和软骨藻酸的初步检测能力。利用重组抗体技术，LOAD平台将免疫荧光与离心驱动的微流控液体处理相结合，以实现一种能够进行多分析物采样的下一代一次性装置。为该平台量身定制了一种基于“发光二极管 - 光电二极管”的低复杂度光学传感系统，该系统可对毒素特异性反应的荧光信号进行定量。该系统能够在30分钟内快速准确地检测微囊藻毒素 - LR、软骨藻酸和石房蛤毒素的存在，最终用户交互最少少于5分钟，以实现最大的重现性。该方法为当前用于定性毒素监测的繁琐且昂贵的方法提供了一种强大的“按需检测”诊断替代方案。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5082/6646042/910a76b2149e/ao-2018-00240x_0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5082/6646042/efdd4560d8a5/ao-2018-00240x_0001.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5082/6646042/6e0ee3e7be19/ao-2018-00240x_0008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5082/6646042/33cac7ba003e/ao-2018-00240x_0009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5082/6646042/69f4913482f7/ao-2018-00240x_0010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5082/6646042/910a76b2149e/ao-2018-00240x_0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5082/6646042/efdd4560d8a5/ao-2018-00240x_0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5082/6646042/75c1a63c5815/ao-2018-00240x_0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5082/6646042/d8551f5def15/ao-2018-00240x_0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5082/6646042/385967ef951b/ao-2018-00240x_0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5082/6646042/0126449656fe/ao-2018-00240x_0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5082/6646042/73f93e4101a7/ao-2018-00240x_0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5082/6646042/6e0ee3e7be19/ao-2018-00240x_0008.jpg
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