Oladosu Olatunbosun Waliu, Biliaminu Sikiru Abayomi, Abdulazeez Ishola Musbau, Nwadike Victor Ugochukwu, Yusuff Jameelu-Deen Omokunmi, Okesina Adekunle Bashiru
Department of Pathology, Federal Medical Centre, Abeokuta, Ogun State, Nigeria.
Department of Chemical Pathology, University of Ilorin, Kwara State, Nigeria.
Niger Postgrad Med J. 2018 Apr-Jun;25(2):94-99. doi: 10.4103/npmj.npmj_51_18.
Seminal fluid analysis (SFA) is the most important investigation of infertile males. It is however limited in revealing the aetiologies of the various spermatozoa abnormalities observed on microscopy. Increasing prevalence of male infertility and the challenges of diagnosis require biochemical investigations, such as assay of seminal malondialdehyde (MDA), a marker of sperm peroxidation and to support SFA in investigation of infertile males.
The aim of this study was to determine the seminal level of biomarker of lipid peroxidation by measuring seminal MDA levels among infertile males in Ilorin.
This was a descriptive cross-sectional study.
One hundred and thirty infertile males served as patients and 50 fertile males as controls. Seminal MDA was assayed using ELISA method.
Statistical Package for the Social Sciences (SPSS) version 20.0 (SPSS Inc., Chicago, IL, USA) was used. Normally distributed data were expressed as mean ± standard deviation.
The mean age of the patients who were mainly civil servants was 38.6 ± 6.6 years compared to 37.0 ± 5.8 years among controls. Seminal MDA was significantly higher among infertile males in this study (P < 0.05) indicating higher degree of lipid peroxidation in their semen. Seminal MDA concentrations were found to be significantly higher among infertile males with a history of alcohol consumption (P < 0.05), cigarette smoking (P < 0.001) and sexually transmitted infections (P < 0.001) when compared to control with similar history.
There is significant lipid peroxidation of spermatozoa among infertile males with lifestyle playing a significant role. Evaluation of infertile males should include assessment of seminal MDA. Prophylactic administration of antioxidants to this group of patients may be beneficial.
精液分析（SFA）是对不育男性最重要的检查。然而，它在揭示显微镜下观察到的各种精子异常的病因方面存在局限性。男性不育患病率的上升以及诊断的挑战需要进行生化检查，例如检测精液丙二醛（MDA），这是一种精子过氧化的标志物，以辅助精液分析用于不育男性的检查。
本研究的目的是通过测量伊洛林不育男性的精液MDA水平来确定脂质过氧化生物标志物的精液水平。
这是一项描述性横断面研究。
130名不育男性作为患者，50名有生育能力的男性作为对照。采用酶联免疫吸附测定（ELISA）法检测精液MDA。
使用社会科学统计软件包（SPSS）20.0版（美国伊利诺伊州芝加哥市SPSS公司）。正态分布的数据以均值±标准差表示。
主要为公务员的患者平均年龄为38.6±6.6岁，而对照组为37.0±5.8岁。本研究中不育男性的精液MDA显著更高（P<0.05），表明其精液中脂质过氧化程度更高。与有类似病史的对照组相比，有饮酒史（P<0.05）、吸烟史（P<0.001）和性传播感染史（P<0.001）的不育男性的精液MDA浓度显著更高。
不育男性精子存在显著的脂质过氧化，生活方式起重要作用。对不育男性的评估应包括精液MDA的检测。对这类患者预防性给予抗氧化剂可能有益。
