Ajina T, Sallem A, Haouas Z, Mehdi M
Histology-Embryology Laboratory, Faculty of Medicine, Monastir, Tunisia.
Cytogenetic and Reproductive Biology Department, Fattouma Bourguiba Teaching Hospital, Monastir, Tunisia.
Andrologia. 2017 Sep;49(7). doi: 10.1111/and.12703. Epub 2016 Sep 29.
The aim of this study was to assess the total antioxidant capacity (TAC) and malondialdehyde (MDA) level in infertile men with asthenozoospermia and asthenoteratozoospermia compared to fertile donors, and to examine the effect of zinc on sperm lipid peroxidation and antioxidant status in infertile and fertile men. Semen samples provided by infertile men (n = 38) and fertile donors (controls; n = 12) were exposed to 6 mmol/L of zinc for 2 hr at 37°C. After semen analysis, lipid peroxidation was detected by MDA assay and seminal TAC was assessed by colorimetric method using TAS (total antioxidant status) Kit. TAC was significantly lower in infertile group compared to controls (p = .037). However, lipid peroxidation did not alter in infertile patients compared to controls (p > .05). After in vitro incubation of samples with zinc, a significant increase in TAC level was found only in infertile men (p < .001). Meanwhile, zinc had no effect on sperm lipid peroxidation in both fertile and infertile men (p > .05). Our data indicate that antioxidant treatment based on zinc in vitro supplementation may be helpful to enhance the rate of seminal antioxidant status in infertile men; however, it does not prevent sperm lipid peroxidation.
本研究旨在评估弱精子症和弱畸精子症不育男性与可育供者相比的总抗氧化能力（TAC）和丙二醛（MDA）水平，并研究锌对不育和可育男性精子脂质过氧化和抗氧化状态的影响。不育男性（n = 38）和可育供者（对照组；n = 12）提供的精液样本在37°C下暴露于6 mmol/L的锌中2小时。精液分析后，通过MDA测定法检测脂质过氧化，并使用TAS（总抗氧化状态）试剂盒通过比色法评估精液TAC。与对照组相比，不育组的TAC显著降低（p = .037）。然而，与对照组相比，不育患者的脂质过氧化没有改变（p > .05）。样本与锌进行体外孵育后，仅在不育男性中发现TAC水平显著升高（p < .001）。同时，锌对可育和不育男性的精子脂质过氧化均无影响（p > .05）。我们的数据表明，基于锌体外补充的抗氧化治疗可能有助于提高不育男性精液的抗氧化状态；然而，它并不能预防精子脂质过氧化。
