Harder P A, Kunz D A
J Bacteriol. 1986 Feb;165(2):650-3. doi: 10.1128/jb.165.2.650-653.1986.
Transformation of Pseudomonas putida and analysis for plasmid DNA revealed that both n-alkane oxidation and mercury resistance are encoded on a single 220-megadalton OCT plasmid molecule. Derivatives of OCT having lost the mercury resistance function could be readily isolated and contained a smaller plasmid estimated to be 170 megadaltons. The results show that segregation of the mercury resistance property occurs not by loss of a separate MER plasmid as previously thought but by a deletion in the OCT plasmid.
恶臭假单胞菌的转化及质粒DNA分析表明，正烷烃氧化和抗汞性均由一个单一的220兆道尔顿的OCT质粒分子编码。失去抗汞功能的OCT衍生物很容易分离得到，并且含有一个估计为170兆道尔顿的较小质粒。结果表明，抗汞特性的分离并非如先前认为的那样是由于一个单独的MER质粒的丢失，而是由于OCT质粒中的一个缺失。
