Gopalakrishnan A S, Chen Y C, Temkin M, Dowhan W
J Biol Chem. 1986 Jan 25;261(3):1329-38.
This paper presents definitive results which establishes a direct gene-protein product relationship between the pgsA gene and the phosphatidylglycerophosphate synthase of Escherichia coli. The predicted protein sequence derived from the determined DNA sequence of pgsA is in close agreement with the amino acid composition and partially determined amino acid sequence of the purified enzyme. The purified synthase has the same apparent molecular mass as the gene product made by a plasmid-directed transcription-translation system. The plasmid-borne copy of the pgsA gene is also capable of expressing enzymatically active synthase in vitro. The DNA sequence analysis has established the exact linear relationship between the uvrC, pgsA, and glyW loci and revealed that these three genes are transcribed in the same direction. The terminal coding regions of these three genes also share common sequences with transcriptional regulatory elements for the adjacent genes.
本文给出了确凿的结果，确立了大肠杆菌pgsA基因与磷脂酰甘油磷酸合酶之间直接的基因-蛋白质产物关系。从pgsA的测定DNA序列推导的预测蛋白质序列与纯化酶的氨基酸组成和部分测定的氨基酸序列高度一致。纯化的合酶与质粒导向的转录-翻译系统产生的基因产物具有相同的表观分子量。pgsA基因的质粒携带拷贝在体外也能够表达具有酶活性的合酶。DNA序列分析确定了uvrC、pgsA和glyW基因座之间确切的线性关系，并揭示这三个基因按相同方向转录。这三个基因的末端编码区也与相邻基因的转录调控元件共享共同序列。
