Papoian R, Pillarisetty R, Talal N
Immunology. 1977 Jan;32(1):75-9.
Serial serum samples from NZB/NZW F1 mice were studied by sucrose density gradient ultracentrifugation and filter radioimmunoassay for the immunoglobulin class of antibodies to double-stranded DNA and single-stranded RNA (Poly A). These antibodies occur spontaneously during the course of an autoimmune disease resembling systemic lupus erythematosus. Study of serum samples at 2-week intervals showed the sequential appearance of 19S followed by 7S anti-nucleic acid antibodies, an earlier commitment to 7S antibodies in female compared to male mice, and an independent and unrelated switching mechanism (19S-7S) for antibodies to DNA and Poly A. The more severe disease in female mice is correlated with an earlier appearance of 7S anti-DNA antibodies. This sequential and ordered appearance of antibodies to nucleic acids is discussed in relation to possible immunologic regulatory mechanisms.
通过蔗糖密度梯度超速离心法和滤膜放射免疫分析法，对NZB/NZW F1小鼠的系列血清样本进行了研究，以检测针对双链DNA和单链RNA（聚腺苷酸）的抗体的免疫球蛋白类别。这些抗体在一种类似系统性红斑狼疮的自身免疫性疾病过程中自发出现。每隔2周对血清样本进行研究，结果显示先是出现19S抗核酸抗体，随后是7S抗核酸抗体；与雄性小鼠相比，雌性小鼠更早出现7S抗体；针对DNA和聚腺苷酸的抗体存在独立且不相关的转换机制（19S - 7S）。雌性小鼠中更严重的疾病与7S抗DNA抗体更早出现相关。结合可能的免疫调节机制，对核酸抗体这种相继且有序的出现情况进行了讨论。
