Department Rehabilitation Sciences, College of Health Sciences, University of Kentucky, 900 S. Limestone, RM 210E, Lexington, KY, 40536-0200, USA.
Center for Muscle Biology, University of Kentucky, Lexington, KY, 40536-0200, USA.
J Muscle Res Cell Motil. 2018 Apr;39(1-2):35-40. doi: 10.1007/s10974-018-9496-x. Epub 2018 Jul 26.
RNA-binding motif protein 3 (RBM3), a stress-inducible RNA-binding protein that increases protein synthesis and confers cell protection in multiple cell types, has been identified as a possible regulator of skeletal muscle mass. Therefore, the primary aim of this study was to examine the impact of elevated RBM3 on skeletal muscle hypertrophy and resistance to atrophy. Plasmid-mediated overexpression of RBM3 in vitro and in vivo was used to assess the role of RBM3 in muscle. CC myotubes overexpressing RBM3 were approximately 1.6 times larger than non-transfected myotubes, suggesting a role for RBM3 in hypertrophy. In addition, elevated RBM3 attenuated atrophy in myotubes exposed to dexamethasone. In agreement with in vitro results, overexpression of RBM3 in soleus muscle of F344/BN rats using electroporation techniques increased the cross sectional area of muscle fibers. Overexpression of RBM3 also attenuated muscle atrophy in rat soleus muscle undergoing disuse atrophy. These findings provide direct evidence for a novel role of RBM3 in inducing hypertrophy as well as attenuating atrophy.
RNA 结合基序蛋白 3（RBM3）是一种应激诱导的 RNA 结合蛋白，可增加多种细胞类型的蛋白质合成并赋予细胞保护作用，它被认为是调节骨骼肌质量的一个潜在调控因子。因此，本研究的主要目的是研究 RBM3 水平升高对骨骼肌肥大和抗萎缩的影响。体外和体内质粒介导的 RBM3 过表达用于评估 RBM3 在肌肉中的作用。过表达 RBM3 的 CC 肌管比未转染的肌管大约大 1.6 倍，表明 RBM3 在肥大中起作用。此外，RBM3 水平升高可减轻肌管中接受地塞米松处理后的萎缩。与体外结果一致，使用电穿孔技术在 F344/BN 大鼠的比目鱼肌中过表达 RBM3 增加了肌纤维的横截面积。过表达 RBM3 还可减轻大鼠比目鱼肌在废用性萎缩过程中的肌肉萎缩。这些发现为 RBM3 在诱导肥大以及减轻萎缩中的新作用提供了直接证据。
