Genetics of Prokaryotes, Faculty of Biology & Center for Biotechnology (CeBiTec), Bielefeld University, Universitaetsstraße 25, Bielefeld, Germany.
Genetics of Prokaryotes, Faculty of Biology & Center for Biotechnology (CeBiTec), Bielefeld University, Universitaetsstraße 25, Bielefeld, Germany.
Bioresour Technol. 2018 Jan;247:744-752. doi: 10.1016/j.biortech.2017.09.167. Epub 2017 Sep 27.
Corynebacterium glutamicum is used for production of the food and feed amino acids l-glutamate and l-lysine at the million-ton-scale. One feed formulation of l-lysine simply involves spray-drying of the fermentation broth, thus, including secreted l-lysine and C. glutamicum cells which are pigmented by the C50 carotenoid decaprenoxanthin. C. glutamicum has been engineered for overproduction of various compounds including carotenoids. In this study, C. glutamicum was engineered for coproduction of a secreted amino acid with a cell-bound carotenoid. Asa proof of principle, coproduction of l-glutamate with the industrially relevant astaxanthin was shown. This strategy was applied to engineer l-lysine overproducing strains for combined overproduction of secreted l-lysine with the cell-bound carotenoids decaprenoxanthin, lycopene, β-carotene, zeaxanthin, canthaxanthin and astaxanthin. By fed-batch fermentation 48g/Ll-lysine and 10mg/L astaxanthin were coproduced. Moreover, C. glutamicum was engineered for coproduction of l-lysine and β-carotene from xylose and arabinose as alternative feedstocks.
谷氨酸棒杆菌用于生产食品和饲料用氨基酸 l-谷氨酸和 l-赖氨酸，产量达到百万吨级。 l-赖氨酸的一种饲料配方只需将发酵液喷雾干燥，因此包括分泌的 l-赖氨酸和被 C50 类胡萝卜素脱甲质叶黄素染色的谷氨酸棒杆菌细胞。为了生产包括类胡萝卜素在内的各种化合物，已经对谷氨酸棒杆菌进行了工程改造。在这项研究中，对谷氨酸棒杆菌进行了工程改造，以共生产一种分泌型氨基酸和一种细胞结合型类胡萝卜素。作为原理的证明，展示了与工业相关的虾青素与 l-谷氨酸的共生产。该策略应用于工程化赖氨酸高产菌株，以共生产分泌型赖氨酸和细胞结合型类胡萝卜素脱甲质叶黄素、番茄红素、β-胡萝卜素、玉米黄质、角黄素和虾青素。通过分批补料发酵，共生产了 48g/L 的 l-赖氨酸和 10mg/L 的虾青素。此外，还对谷氨酸棒杆菌进行了工程改造，使其能够从木糖和阿拉伯糖共生产 l-赖氨酸和 β-胡萝卜素作为替代饲料。
