用于单纯疱疹病毒分离的原代兔肾细胞和MRC-5细胞培养物的商业来源比较。

Comparison of commercial sources of primary rabbit kidney and MRC-5 cell cultures for herpes simplex virus isolation.

作者信息

Smith C A, Rubin S J

出版信息

Diagn Microbiol Infect Dis. 1986 Feb;4(2):147-52. doi: 10.1016/0732-8893(86)90149-5.

Abstract

Two commercial sources of MRC-5 and primary rabbit kidney cell cultures were compared for sensitivity of herpes simplex virus isolation and cytopathic effect detection time. Forty-six of 78 previously positive and 50 of 234 fresh clinical specimens were positive and could be evaluated. Herpes simplex virus recovery from frozen specimens positive in at least one cell culture ranged from 85% in MRC-5 cells (M.A. Bioproducts) to 89% in primary rabbit kidney cells (M.A. Bioproducts). Herpes simplex virus recovery from fresh clinical specimens ranged from 94% in primary rabbit kidney cells (Flow Laboratories), MRC-5 cells (Flow Laboratories), and MRC-5 (M.A. Bioproducts) cells to 100% in primary rabbit kidney (M.A. Bioproducts) cells. Among the 96 positive specimens evaluated, no significant difference was found between commercially prepared MRC-5 and primary rabbit kidney cell cultures in either sensitivity or time to first detectable cytopathic effect. Cells from M.A. Bioproducts and Flow Laboratories were equally sensitive. Herpes simplex virus detection was dependent upon inoculating at least two cell culture tubes rather than if the cells were MRC-5 cells or primary rabbit kidney cells.

摘要

对两种商业来源的MRC-5细胞和原代兔肾细胞培养物进行了比较，以观察单纯疱疹病毒分离的敏感性和细胞病变效应检测时间。在78份先前呈阳性的样本中有46份以及234份新鲜临床样本中有50份呈阳性，且可进行评估。在至少一种细胞培养中呈阳性的冷冻样本中，单纯疱疹病毒的回收率在MRC-5细胞（M.A. Bioproducts公司）中为85%，在原代兔肾细胞（M.A. Bioproducts公司）中为89%。新鲜临床样本中单纯疱疹病毒的回收率在原代兔肾细胞（Flow Laboratories公司）、MRC-5细胞（Flow Laboratories公司）和MRC-5（M.A. Bioproducts公司）细胞中为94%，在原代兔肾（M.A. Bioproducts公司）细胞中为100%。在评估的96份阳性样本中，市售的MRC-5细胞和原代兔肾细胞培养物在敏感性或首次检测到细胞病变效应的时间方面均未发现显著差异。M.A. Bioproducts公司和Flow Laboratories公司的细胞同样敏感。单纯疱疹病毒的检测取决于接种至少两支细胞培养管，而不是取决于细胞是MRC-5细胞还是原代兔肾细胞。