Zhao L S, Landry M L, Balkovic E S, Hsiung G D
J Clin Microbiol. 1987 Aug;25(8):1401-5. doi: 10.1128/jcm.25.8.1401-1405.1987.
Tremendous interest has been generated in the commercial kits now available that incorporate herpes simplex virus isolation in cell culture with immunoperoxidase staining for viral antigen detection. Most studies comparing commercial kits with conventional cell culture techniques have found the kits to be less sensitive. However, different cell cultures were used for the two methods. In this study, mink lung, rabbit kidney, MRC-5, and Vero cells were compared for reisolation of herpes simplex virus from clinical specimens in which viral infectivity titers were concurrently determined. When specimens contained high titers of infectious virus, the cell system used made little difference and all specimens were detected by immunoperoxidase staining at 48 h postinoculation. However, when specimens contained low concentrations of virus, the differences in sensitivity between cell systems became apparent in rapidity of detection and overall isolation rate. Mink lung and rabbit kidney cells were both more sensitive than MRC-5 cells; Vero cells were significantly less sensitive than the other cells tested. The application of immunoperoxidase staining shortened the time to virus detection and lessened, but did not eliminate, the differences between the cell systems. Cytopathic effects alone in the most sensitive cell system equaled or exceeded immunoperoxidase staining applied in less-sensitive cell cultures.
目前市面上有一些商业试剂盒，它们将单纯疱疹病毒在细胞培养中的分离与用于病毒抗原检测的免疫过氧化物酶染色结合在一起，引发了人们极大的兴趣。大多数将商业试剂盒与传统细胞培养技术进行比较的研究发现，这些试剂盒的敏感性较低。然而，这两种方法使用了不同的细胞培养物。在本研究中，比较了貂肺细胞、兔肾细胞、MRC - 5细胞和Vero细胞从临床标本中再次分离单纯疱疹病毒的情况，同时测定了病毒感染滴度。当标本含有高滴度的感染性病毒时，所使用的细胞系统差异不大，所有标本在接种后48小时通过免疫过氧化物酶染色均可检测到。然而，当标本含有低浓度病毒时，细胞系统之间在检测速度和总体分离率方面的敏感性差异就变得明显了。貂肺细胞和兔肾细胞都比MRC - 5细胞更敏感；Vero细胞的敏感性明显低于其他测试细胞。免疫过氧化物酶染色的应用缩短了病毒检测时间，减少了但并未消除细胞系统之间的差异。在最敏感的细胞系统中，仅细胞病变效应就等于或超过了在敏感性较低的细胞培养物中应用的免疫过氧化物酶染色。
