Clark S H, McCarron M, Love C, Chovnick A
Genetics. 1986 Apr;112(4):755-67. doi: 10.1093/genetics/112.4.755.
DNA extracts of several rosy-mutation-bearing strains were associated with large insertions and deletions in a defined region of the molecular map believed to include the rosy locus DNA. Large-scale, intragenic mapping experiments were carried out that localized these mutations within the boundaries of the previously defined rosy locus structural element. Molecular characterization of the wild-type recombinants provides conclusive evidence that the rosy locus DNA is localized to the DNA segment marked by these lesions. One of the mutations, ry2101, arose from a P-M hybrid dysgenesis experiment and is associated with a copia insertion. Experiments are described which suggest that copia mobilizes in response to P-M hybrid dysgenesis. Relevance of the data to recombination in higher organisms is considered.
几种带有玫瑰色突变的菌株的DNA提取物与分子图谱特定区域的大插入和缺失有关，该区域被认为包含玫瑰色基因座DNA。进行了大规模的基因内定位实验，将这些突变定位在先前定义的玫瑰色基因座结构元件的边界内。野生型重组体的分子特征提供了确凿证据，表明玫瑰色基因座DNA定位于由这些损伤标记的DNA片段。其中一个突变体ry2101来自P-M杂种不育实验，与一个copia插入相关。文中描述的实验表明，copia会响应P-M杂种不育而移动。还考虑了这些数据与高等生物重组的相关性。
